H5041

Cystatin C human

HumanKine^®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, suitable for cell culture

• MDL number: MFCD00163390
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• product name: Cystatin C human, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• biological source: human
• Quality Level: 100
• recombinant: expressed in HEK 293 cells
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤5 μM IC₅₀
• quality: endotoxin tested
• mol wt: dimer 12-13 kDa (non-glycosylated)
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/mg
• UniProt accession no.: P01034
• storage temp.: −20°C
• Gene Information: human ... CYTC(1471)

Description

General description

HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells. Cystatin C belongs to the cystatin superfamily. Two isoforms (pI = 7.8, 9.2) of native Cystatin C are found in human urine differentiated by the elimination of small basic peptides or amino acids from the N-terminal end of the protein. This protein is coded by a housekeeping gene, CST3 located on human chromosome 20p11.21 and is generated by nucleated cells.

Application

Cystatin C human has been used as a standard in western blot assay. It has also been used to study its effect on the endopeptidase activity of activated Trichobilharzia regenti isoform of cathepsin B1 peptidase (TrCB1).

Biochem/physiol Actions

Cystatin C is an inhibitor of cysteine proteases including cathepsin B which has been identified as the most important β-amyloid-degrading enzyme. Measurement of cystatin C in serum is replacing creatinine as an indicator of kidney function (glomerular filtration rate, GFR). Studies show its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer′s disease. Cystatin C inhibits transforming growth factor β signaling in normal and cancer cells.

Physical form

Lyophilized from a 0.2 μm filtered solution of 1×PBS

Analysis Note

The inhibitory function of cystatin c on papain′s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC₅₀ value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56 μg/mL to 50 μg/mL cystatin C in presence of 0.55 μM papain and 0.44 μM L-BAPA.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable