Skip to Content
Merck
All Photos(1)

Key Documents

H2387

SAFC

Hanks′ Balanced Salts

Modified, without calcium chloride, magnesium sulfate and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

HBSS

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

Balanced salt solution (BSS) provides cells with water, bulk inorganic ions, and buffering system to maintain the normal cell metabolism and physiological range (pH 7.2-7.6), respectively. It also acts as an irrigating, diluting, and transporting agent and maintains the intracellular and extracellular osmotic balance. BSS also provides energy sources such as glucose for cell metabolism.

Application

Hanks′ Balanced Salt has been used:

  • to culture primary hippocampal neuron cells from rats
  • to collect the rat brains
  • to culture rat primary hepatocytes

Quantity

Formulated to contain 9.5 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service