G7295

Anti-GM130 (C-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-GM130 (Golgi Matrix Protein of 130 kDa), Anti-Golgi Autoantigen, Anti-Golgi Matrix Protein GM 130
• MDL number: MFCD01633397
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~130 kDa
• species reactivity: mouse, human, rat
• technique(s): immunoprecipitation (IP): 2-4 μg using Hela and 3T3 cell extracts.; indirect immunofluorescence: 0.2-0.4 μg/mL using rat NRK cells; western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of rat NRK cells
• UniProt accession no.: Q08379
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... GOLGA2(2801) ; mouse ... Golga2(99412) ; rat ... Golga2(64528)

Description

General description

Golgi Matrix Protein of 130 kDa (GM130) is a peripheral cytoplasmic protein and is a member of the golgin family of Golgi autoantigens. GM130 is an extended rod-like protein containing coiled-coil domains and is tightly bound to Golgi membranes.

Immunogen

synthetic peptide corresponding to the C-terminal region of human GM130 with an N-terminal added lysine, conjugated to KLH. The corresponding sequence is identical in rat and mouse GM130.

Application

Anti-GM130 (C-terminal) antibody can be used in immunoblotting (approx. 130 kDa), indirect immunofluorescence (0.2-0.4 μg/mL using rat NRK cells), and immunoprecipitation (2-4 μg using Hela and 3T3 cell extracts). The antibody can also be used for chemiluminescent western blot (0.1-0.2 μg/mL using whole extract of rat NRK cells).

Anti-GM130 (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• indirect immunofluorescence
• immunoprecipitation
• immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Golgi Matrix Protein of 130 kDa (GM130) is involved in maintaining the structural integrity of the Golgi apparatus and vesicular transport. GM130 also interacts with Golgi reassembly-stacking protein of 65 kDa (GRASP65) and participated in postmitotic reassembly and stacking of the Golgi cisternae. The protein along with p115 and GRASP65 might acts as a template in nucleating the formation of the Golgi apparatus. GM130 in complex with GRASP65 and other proteins interacts with activated Rab1, a protein known to regulate the transport of newly synthesized proteins from the endoplasmic reticulum (ER) to the Golgi apparatus. This antibody can be used as a cis-Golgi network marker.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7,.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable