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Key Documents

FP0010

Sigma-Aldrich

FluoroProfile Protein Quantification Kit

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About This Item

UNSPSC Code:
12352200

shipped in

wet ice

storage temp.

−20°C

Application

Reversible covalent binding to proteins by the fluorophore epicocconone provides a powerful and sensitive method for protein quantification

Features and Benefits

  • Yields an intense fluorescent red on protein binding
  • Binding is completely reversible making the protein suitable for mass spectrometry and functional assays
  • Water soluble proteins may be quantified without using organic solvents
  • A large dynamic range, large Stokes shift and highly sensitive
  • Highly tolerant of substances commonly interfering with protein quantification
  • Biodegradable and environmentally friendly

Other Notes

For the quantification of total protein in solution by the interaction of a unique fluorophore with the amines of polypeptides.

Legal Information

FluoroProfile is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • P5619Protein Standard, analytical standard, 2 mg/vial BSASDS

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ondřej Kaplan et al.
PloS one, 11(10), e0163697-e0163697 (2016-10-08)
We describe the production of a highly-active mutant VEGF variant, α2-PI1-8-VEGF121, which contains a substrate sequence for factor XIIIa at the aminoterminus designed for incorporation into a fibrin gel. The α2-PI1-8-VEGF121 gene was synthesized, cloned into a pET-32a(+) vector and
Phillip J L Bell et al.
Journal of the American Chemical Society, 125(31), 9304-9305 (2003-08-02)
Epicocconone represents a new class of natural fluorescent probes based on a polyketide skeleton isolated from the fungus Epicoccum nigrum. Epicocconone is a small, cell permeable natural product with a high molar absorbtivity and a long Stokes' shift that will
R P Ubrihien et al.
Archives of environmental contamination and toxicology, 82(4), 520-538 (2022-04-21)
The effects of multigenerational Cu exposure on the freshwater gastropod Isidorella newcombi were investigated. Snails were exposed to a range of treatment-specific Cu concentrations in the parental to F2 generations, and a common Cu concentration in the F3 generation. In
Jiaorong Tan et al.
Journal of diabetes research, 2019, 5483946-5483946 (2019-06-22)
This study is aimed at evaluating the effects, functions, and mechanism of HNF1α on hepatic glycolipid metabolism. In this study, free fatty acid- (FFA-) induced steatosis of hepatocyte liver cell LO2 was used as an in vitro model. The methods
Helena A Schmitz et al.
Aquatic toxicology (Amsterdam, Netherlands), 160, 22-30 (2015-01-13)
Metals are accumulated by filter feeding organisms via water, ingestion of suspended sediments or food. The uptake pathway can affect metal toxicity. Saccostrea glomerata were exposed to cadmium through cadmium-spiked suspended sediments (19 and 93μg/g dry mass) and cadmium-enriched phytoplankton

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