FA20
Fructose Assay Kit
sufficient for 20 assays
Synonym(s):
Fructose Test Kit
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About This Item
Recommended Products
usage
sufficient for 20 assays
Quality Level
analyte chemical class(es)
sugars (fructose)
technique(s)
photometry: suitable
application(s)
food and beverages
general analytical
storage temp.
2-8°C
Application
This kit is for the quantitative, enzymatic determination of fructose in food and other materials.Fructose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Fructose 6-phosphate is converted to glucose 6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in the reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to fructose concentration.
Kit Components Only
Product No.
Description
- fructose standard 500 mg
- glucose assay reagent 1 mL/vial
- phosphoglucose isomerase 1 mL/vial
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Certificates of Analysis (COA)
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Official Methods of Analysis of the AOAC, 28-28 (1995)
Beuter, H.O.
Methods of Enzymatic Analysis, 6, 321-321 (1985)
Chih-Yu Cheng et al.
FEBS letters, 589(20 Pt B), 3107-3112 (2015-09-04)
Laccase and borate-fructose complex were investigated by coincidence in a solid-state fermentation of Edenia sp. TS-76 under fructose oxidase screening. Laccase was purified to homogeneity with a 34-fold purification and 32% yield. Fructose had no significant effect on laccase activity
Yifan Chen et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 36(3), 373-378 (2019-11-19)
Cholesteryl ester (CE) is an ester of cholesterol and fatty acid (FA). Plasma CE reflects complicated metabolisms of cholesterol, phospholipids, lipoproteins, and dietary FAs. An informatics approach could be useful for analysis of the CE species. In this study, two
Mengxuan Fang et al.
Analytical and bioanalytical chemistry, 412(10), 2339-2351 (2020-02-02)
Fatty acids are a major source of structural diversity within the lipidome due to variations in their acyl chain lengths, branching, and cyclization, as well as the number, position, and stereochemistry of double bonds within their mono- and poly-unsaturated species.
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