DUO92104

Duolink^® In Situ Orange Starter Kit Mouse/Goat

• Synonym(s): in situ Proximity Ligation Assay reagent, Protein Protein Interaction Kit
• UNSPSC Code: 41116158
• NACRES: NA.32

Properties

• product line: Duolink^®
• Quality Level: 100
• technique(s): proximity ligation assay: suitable
• fluorescence: λ_ex 554 nm; λ_em 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)
• suitability: suitable for fluorescence

Description

Application

Duolink^®proximity ligation assay(PLA^®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink^® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink^® PLA Resource Center for information on how to run a Duolink^® experiment, applications, troubleshooting, and more.

To perform a complete Duolink^® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. This starter kit supplies all other necessary reagents for 30 Duolink^® PLA reactions, which include a pair of PLA probes (Anti-Mouse PLUS and Anti-Goat MINUS), orange detection reagents, wash buffers, and mounting medium.Note that the primary antibodies must come from the same species as the Duolink^® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
The Duolink^® In Situ Orange Starter Kit Mouse/Goat requires one primary antibody from mouse and one primary antibody from goat. Orange fluorescence detection reagents are often used with Cyanine 3 filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

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Features and Benefits

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

Legal Information

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany

PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

• DUO92006 Duolink^® In Situ PLA^® Probe Anti-Goat MINUS, Affinity purified Donkey anti-Goat IgG (H+L)
• DUO92001 Duolink^® In Situ PLA^® Probe Anti-Mouse PLUS
• DUO92007 Duolink^® In Situ Detection Reagents Orange
• DUO82049 Duolink^® In Situ Wash Buffers, Fluorescence
• DUO82040 Duolink^® In Situ Mounting Medium with DAPI

Safety Information

• Pictograms: GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H290,H317,H334,H411
• Precautionary Statements: P234 - P261 - P272 - P273 - P280 - P302 + P352
• Hazard Classifications: Aquatic Chronic 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable