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Key Documents

D4645

Sigma-Aldrich

Sodium 3,5-dichloro-2-hydroxybenzenesulfonate

≥98% purity (TLC), powder

Synonym(s):

3,5-Dichloro-2-hydroxybenzenesulfonic acid, DCHBS, DHBS

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About This Item

Empirical Formula (Hill Notation):
C6H3Cl2NaO4S
CAS Number:
Molecular Weight:
265.05
Beilstein:
3640643
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Sodium 3,5-dichloro-2-hydroxybenzenesulfonate, used for peroxide measurement

Quality Level

Assay

≥98% (TLC)

form

powder

color

white to off-white

pH

7 (20 °C, 53 g/L)

mp

>300 °C

solubility

H2O: 50 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Oc1c(Cl)cc(Cl)cc1S([O-])(=O)=O

InChI

1S/C6H4Cl2O4S.Na/c7-3-1-4(8)6(9)5(2-3)13(10,11)12;/h1-2,9H,(H,10,11,12);/q;+1/p-1

InChI key

NMWCVZCSJHJYFW-UHFFFAOYSA-M

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General description

Sodium 3,5-dichloro-2-hydroxybenzenesulfonate is used in conjunction with 4-aminoantipyrine (4-AAP) and hydrogen peroxide (H2O2) for chromogenic quantitation of uric acid in serum and urine samples.

Application

Used in conjunction with 4-aminoantipyrine (4-AAP) and hydrogen peroxide (H2O2) for chromogenic quantitation of peroxidase in coupled enzymatic reactions. Component of Trinder reagent for use with peroxidase to measure generation of hydrogen peroxide in automated systems.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone chromogenic system in direct enzymatic assay of uric acid in serum and urine.
Fossati P, et al.
Clinical Chemistry, 26(2), 227-231 (1980)
Teresa Ramon-Marquez et al.
Talanta, 187, 83-90 (2018-06-02)
In this study, we have optimised the sterically directed attachment of biomolecules on the surface of coaxial membranes prepared by co-electrospinning which have been proved to be a material with very high performance for the development of biosensors with optical
P Fossati et al.
Clinical chemistry, 29(8), 1494-1496 (1983-08-01)
We describe a new colorimetric method for measuring creatinine in serum and urine. Creatinine hydrolysis is catalyzed by creatinine amidohydrolase, and the creatine so produced is assayed in reactions catalyzed sequentially by creatine amidinohydrolase and sarcosine oxidase in a system
J D Artiss et al.
Clinica chimica acta; international journal of clinical chemistry, 116(3), 301-309 (1981-11-11)
A uricase-peroxidase coupled system, for the determination of uric acid, applied to a CentrifiChem-500 centrifugal fast analyser is described. Relatively large amounts of ascorbic acid, due to the inclusion of an ascorbate oxidase urine diluent, and hemoglobin appear not to
Seulgi Ju et al.
Frontiers in plant science, 8, 1210-1210 (2017-07-27)
The cuticle of land plants is the first physical barrier to protect their aerial parts from biotic and abiotic stresses. DEWAX, an AP2/ERF-type transcription factor, negatively regulates cuticular wax biosynthesis. In this study, we investigated the resistance to

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