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C9142

Sigma-Aldrich

Cyanogen bromide-activated-Sepharose™ 4B

lyophilized powder

Synonym(s):

Cyanogen bromide-activated Agarose

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About This Item

CAS Number:
68987-32-6
MDL number:
MFCD00163385
UNSPSC Code:
41106500
NACRES:
NA.56

form

lyophilized powder

Quality Level

200

technique(s)

affinity chromatography: suitable

matrix

Sepharose 4B

matrix active group

cyanate or related structures

matrix spacer

1 atom (when ligands are coupled via isourea derivative or related linkage)

swelling

1 g swells to 4-5 mL

particle size

45—165 μm

storage temp.

2-8°C

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General description

Cyanogen bromide-activated-Sepharose™ is suitable for use in affinity chromatography and protein chromatography. It has been used to discover insecticidal agents, raise polyclonal antibodies against yeast invertase glycosyls, and study immunological processes of xenograft rejection.

C9142-15G′s updated product number is GE17-0430-01

Application

Cyanogen bromide-activated-Sepharose™ 4B has been used:
  • in the purification of clusterin from serum by an immunoaffinity-based separation procedure
  • in affinity chromatography to isolate Leishmania donovani protein antigens from the urine of visceral leishmaniasis patients
  • in affinity chromatography for the isolation of haptoglobin from human serum

Physical form

Lyophilized powder stabilized with lactose and dextran

Legal Information

Sepharose is a trademark of Cytiva

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hans Gaus et al.
Biochemistry, 57(14), 2061-2064 (2018-03-29)
The Stabilin receptors are systemic clearance receptors for some classes of chemically modified nucleic acid therapeutics. In this study, the recombinant human secreted ecto-domain of the small isoform of Stabilin-2 (s190) was purified from cell culture and evaluated for direct
Maia Dorsett et al.
Molecular and cellular biology, 29(22), 6128-6139 (2009-09-16)
During normal development as well as in diseased states such as cancer, extracellular "niches" often provide cues to proximal cells and activate intracellular pathways. Activation of such signaling pathways in turn instructs cellular proliferation and differentiation. In the Caenorhabditis elegans
Kevin K Takaki et al.
Cell host & microbe, 29(1), 58-67 (2020-10-30)
Schistosome eggs provoke the formation of granulomas, organized immune aggregates, around them. For the host, the granulomatous response can be both protective and pathological. Granulomas are also postulated to facilitate egg extrusion through the gut lumen, a necessary step for
Mark A Poli et al.
Journal of AOAC International, 90(1), 173-178 (2007-03-22)
A new competitive electrochemiluminescence-based immunoassay for the type-2 brevetoxins in oyster extracts was developed. The assay was verified by spiking known amounts of PbTx-3 into 80% methanol extracts of Gulf Coast oysters. We also provide preliminary data demonstrating that 100%
R S Koduri et al.
The Journal of biological chemistry, 273(48), 32142-32153 (1998-11-21)
Human group IIa phospholipase A2 (hIIa-PLA2) is a highly basic protein that is secreted from a number of cells during inflammation and may play a role in arachidonate liberation and in destruction of invading bacteria. It has been proposed that
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