Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

C0662

Sigma-Aldrich

6-Carboxyfluorescein

~97% purity (HPLC), powder

Synonym(s):

6-FAM, FLUOS

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C21H12O7
CAS Number:
3301-79-9
Molecular Weight:
376.32
Beilstein:
54341
MDL number:
MFCD00036873
UNSPSC Code:
12171500
PubChem Substance ID:
24892266
NACRES:
NA.47

product name

6-Carboxyfluorescein, ≥96% (HPLC)

Quality Level

200

Assay

≥96% (HPLC)

form

powder

technique(s)

titration: suitable

color

dark yellow to dark orange

mp

>300 °C (lit.)

solubility

0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy, orange

λmax

492 nm at 0.1 N in NaOH

fluorescence

λex 492 nm; λem 517 nm (pH 8.0)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

OC(=O)c1ccc2C(=O)OC3(c4ccc(O)cc4Oc5cc(O)ccc35)c2c1

InChI

1S/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key

BZTDTCNHAFUJOG-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

6-Carboxyfluorescein has been used to check the plasma membrane integrity in sperm cells.
Fluorescent dye.

Biochem/physiol Actions

6-Carboxyfluorescein diacetate is a membrane permeable compound. It can be hydrolysed by intracellular esterases to form a membrane impermeable bright green fluorescent molecule, 6-carboxyfluorescein.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Przemysław Płociński et al.
Nature communications, 8(1), 1251-1251 (2017-11-02)
Prokaryotic Ligase D is a conserved DNA repair apparatus processing DNA double-strand breaks in stationary phase. An orthologous Ligase C (LigC) complex also co-exists in many bacterial species but its function is unknown. Here we show that the LigC complex
Andrew B Janowski et al.
mBio, 10(4) (2019-07-11)
Recent advances in unbiased pathogen discovery have implicated astroviruses as pathogens of the central nervous system (CNS) of mammals, including humans. However, the capacity of astroviruses to be cultured in CNS-derived cells in vitro has not been reported to date.
Bikash Malla et al.
Food and environmental virology, 11(3), 274-287 (2019-05-16)
Monitoring of environmental water is crucial to protecting humans and animals from possible health risks. Although numerous human-specific viral markers have been designed to track the presence of human fecal contamination in water, they lack adequate sensitivity and specificity in
Comparison of efficiency between two artificial insemination methods using frozen-thawed semen in domestic cat (Felis catus): artificial insemination in domestic cats.
Villaverde AI, et al.
Animal Reproduction Science, 114, 434-434 (2009)
Sofia Persson et al.
Food and environmental virology, 11(3), 297-308 (2019-04-21)
Mismatches between template sequences and reverse transcription (RT) or polymerase chain reaction (PCR) primers can lead to underestimation or false negative results during detection and quantification of sequence-diverse viruses. We performed an in silico inclusivity analysis of a widely used
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service