B7542
Maleimide Activated BSA
Synonym(s):
Maleimide Activated Bovine Serum Albumin
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About This Item
UNSPSC Code:
12352111
NACRES:
NA.32
Recommended Products
biological source
bovine
Quality Level
sterility
non-sterile
form
lyophilized powder
technique(s)
ELISA: suitable
bioconjugation: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
bovine ... ALB(280717)
General description
Maleimide Activated BSA is provided in lyophilized, ready to-use form, eliminating the need for time-consuming activation and purification steps of the carrier protein. Maleimide Activated BSA contains approximately 5-15 maleimide groups for each BSA molecule.. The conjugation reaction with a cysteine containing peptide is performed at pH 6.6-7.0. The extent of conjugation can then be determined by a colorimetric assay with Ellman′s reagent) to detect unreacted thiol groups in the cysteine-containing peptide.
Application
Maleimide Activated BSA has been used in peptide conjugation.
Maleimide-activated BSA (mBSA) has reactive maleimide groups on its surface available for conjugation with a cysteine-containing peptide or a thiol-containing hapten. The reaction of the maleimide group and the thiol group proceeds rapidly and selectively under mild coupling conditions (pH 6.5-7.5) to yield a stable, covalently linked peptide-protein conjugate that may be used in immunization protocols or in antibody screening methods.
Physical form
Each vial contains 5 mg of Maleimide Activated BSA lyophilized from 10 mM sodium phosphate buffer, pH 6.6, with 0.115 mM NaCl, 1 mM EDTA, and 40 mM sucrose as stabilizer.
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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