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A6792

Sigma-Aldrich

Anti-Dog IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Rabbit Anti-Dog IgG (whole molecule)−HRP

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

IgG (immunoglobulin G) antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Mammalian IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c.
Specificity of the anti-dog IgG antiserum is determined by immunoelectrophoresis, prior to conjugation, versus normal dog serum and dog IgG.

Application

Anti-Dog IgG (whole molecule) Peroxidase antibody produced in rabbit has been used:
  • for the detection of antibodies against noro viruses, by ELISA (enzyme linked immunosorbent asay) at 1:2000 for 1 hour at 37°C
  • in IgG (Immunoglobulin G) staining method
  • in ELISA to detect the incorporation of RVGTM (a rabies virus G protein variant)

Biochem/physiol Actions

IgG (Immunoglobulin G) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. Anti-Dog IgG antibody is a secondary antibody. Second antibodies (secondary antibodies) are raised against primary antibodies, which bind to specific antigens to create antigen antibody complexes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 0.05% MIT

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Detection of antibodies against norovirus genogroup GIV in carnivores
Di MB, et al.
Clinical and Vaccine Immunology : CVI, 17(1), 180-182 (2010)
The laboratory rat
The Laboratory Rat (2005)
Juliano Bordignon et al.
Revista do Instituto de Medicina Tropical de Sao Paulo, 44(3), 151-154 (2002-08-07)
The determination of the rabies neutralizing antibody (VNA) response after immunization against rabies is an acceptable index of the efficacy of a vaccine and a successful treatment. Several tests have been developed in attempt to improve the assessment of VNA
Lukas Uhrik et al.
The Biochemical journal, 478(1), 99-120 (2020-12-08)
A comparative canine-human therapeutics model is being developed in B-cell lymphoma through the generation of a hybridoma cell that produces a murine monoclonal antibody specific for canine CD20. The hybridoma cell produces two light chains, light chain-3, and light chain-7.
Vivian Tamietti Martins et al.
PloS one, 10(9), e0137683-e0137683 (2015-09-15)
In the present study, two Leishmania infantum hypothetical proteins present in the amastigote stage, LiHyp1 and LiHyp6, were combined with a promastigote protein, IgE-dependent histamine-releasing factor (HRF); to compose a polyproteins vaccine to be evaluated against L. infantum infection. Also

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