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Key Documents

A4800

Sigma-Aldrich

Agar

Type M, suitable for plant cell culture

Synonym(s):

Agar-agar, Gum agar

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About This Item

Linear Formula:
(C12H18O9)n
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
50405901
eCl@ss:
42040102
NACRES:
NA.21

biological source

algae (Rhodophyceae)

Quality Level

type

Type M

form

powder

technique(s)

cell culture | plant: suitable

transition temp

transition temp 34-36 °C

application(s)

agriculture

storage temp.

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

InChI key

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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General description

Type M agar is predominantly used in plant tissue culture. It is similar to agar, a polysaccharide complex with a backbone of alternating agarose and agaropectin molecules and extracted from red algae.

Application

Type M agar has been used as a component of Murashige and Skoog (MS) medium for culturing Arabidopsis thalania seeds. It has also been used in rooting media for tomato (Solanum lycopersicum) seeds.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sanjaya B Ekanayake et al.
Methods in molecular biology (Clifton, N.J.), 1305, 223-240 (2015-04-26)
One of the most striking features of plant mitochondria when visualized in living tissue is their dynamism. The beauty of cytoplasmic streaming, driving, and being driven by the motility of mitochondria and other small organelles belies the complexity of the
Xiaohua Wang et al.
Nature protocols, 10(12), 2054-2063 (2015-11-20)
Measuring the mobility and interactions of proteins is key to understanding cellular signaling mechanisms; however, quantitative analysis of protein dynamics in living plant cells remains a major challenge. Here we describe an automated, single-molecule protocol based on total internal reflection
Sangeeta Negi et al.
The Plant journal : for cell and molecular biology, 61(1), 3-15 (2009-10-02)
In this study we investigated the role of ethylene in the formation of lateral and adventitious roots in tomato (Solanum lycopersicum) using mutants isolated for altered ethylene signaling and fruit ripening. Mutations that block ethylene responses and delay ripening -Nr
Extraction and characterization of agar from Australian Pterocladia lucida
Chiovitti A, et al.,
Journal of Applied Phycology, 16(1), 41-48 (2004)
GunNam Na et al.
The Journal of biological chemistry, 286(47), 40423-40432 (2011-09-21)
When growing in its native habitat, Thlaspi goesingense can hyperaccumulate 1.2% of its shoot dry weight as nickel. We reported previously that both constitutively elevated activity of serine acetyltransferase (SAT) and concentration of glutathione (GSH) are involved in the ability

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