A4062

Anti-Goat IgG (whole molecule)–Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): Rabbit Anti-Goat IgG (whole molecule)–AP
• MDL number: MFCD00162335
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• species reactivity: goat
• technique(s): direct ELISA: 1:30,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50; western blot: 1:30,000
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulins (Igs) belongs immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobin have two heavy (H) and two light (L), held together by disulphide linkages. Each light comprises of one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.

Immunogen

purified goat IgG

Application

Alkaline phosphatase conjugated rabbit anti goat IgG was used as the secondary for ELISA assays to detect the binding of bacterial proteins to C components. The antibody was used at 1:5000 dilutions and detected using P-nitrophenyl phosphate at 1mg/ml (Sigma) as the substrate.

Anti-Goat IgG (whole molecule)-alkaline phosphatase antibody can be used in western blotting and immunoblotting (diluted 1: 20,000) .

Biochem/physiol Actions

IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus, they play a crucial role in facilitating cytological immune responses. Anti-goat IgG (whole molecule)-alkaline phosphatase antibody (diluted 1: 5000) can be used for anti-OVA IgG detection. Rabbit anti- goat IgG (whole molecule)-alkaline phosphatase antibody reacts specifically with all goat immunoglobulins.

Other Notes

Antibody adsorbed with human serum proteins.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 10 mM glycine, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide

Preparation Note

Adsorbed to reduce background staining with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves