A3672

Azurin

from Pseudomonas aeruginosa, lyophilized powder

• CAS Number: 12284-43-4
• EC Number: 235-551-8
• MDL number: MFCD00130594
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: Pseudomonas aeruginosa
• Quality Level: 200
• form: lyophilized powder
• composition: Protein, ≥65% Lowry
• concentration: ≥65.0% (Lowry)
• technique(s): toxicology assay: suitable
• solubility: water: soluble 1—1.1 mg/mL, clear, blue (light blue to blue)
• UniProt accession no.: P00282
• storage temp.: −20°C
• Gene Information: Pseudomonas aeruginosa ... AZU(878046)

Description

General description

Research area: Apoptosis. Azurinis a periplasmic protein and is a homotetramer.

Application

Azurin has been used:

• in the cytotoxicity and cell viability studies in human osteosarcoma cell line
• for the functionalization of silicon nitride cantilevers for interaction studies
• for coating gold surface and insulating functionalized oxide surfaces of silicon oxide and mica

Biochem/physiol Actions

Azurin acts as an electron donor for nitrite reductase in bacterial denitrification process. It exhibits anticancer activity as it hampers various independent signaling pathways associated with cancer progression. It binds to tumor suppressor protein p53 and induces cancer cell apoptosis or stalls cancer cell growth. Azurin disrupts angiogenesis by reducing the activity of VEGFR-2tyrosine kinase thereby inhibiting tumor growth. It has been observed to show cytotoxicity in human breast cancer cells and human melanoma cells.

Azurin is a metalloprotein in the family of cupredoxins. It preferentially enters cancer cells over normal cells and induces apoptosis. Azurin has structural similarities to ephrinB2, and in fact binds the ephrin receptor tyrosine kinase EphB2 to initiate cell signaling that is involved in cancer progression. Azurin inhibits autophosphorlyation of the EphB2 tyrosine residue, interfering with upstream cell signaling and contributing to cancer cell growth inhibition.

Physical form

Lyophilized powder containing ammonium acetate buffer salts.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves