A2831
Monoclonal Anti-DNP−Alkaline Phosphatase Conjugate antibody produced in mouse
clone SPE-7, purified immunoglobulin, buffered aqueous glycerol solution
Synonym(s):
Anti-Dinitrophenyl, Monoclonal Anti-DNP
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.46
Recommended Products
biological source
mouse
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
SPE-7, monoclonal
form
buffered aqueous glycerol solution
technique(s)
direct ELISA: 1:25,000 using DNP-BSA
dot blot: 1:180,000 (chemiluminescence)
isotype
IgE
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Looking for similar products? Visit Product Comparison Guide
General description
Dinitrophenyl (DNP) is a hapten that is used for labeling primary or secondary probes in immunological analyses. Thus, anti-DNP antibody conjugated to a detectable substrate is a useful tool for the study of target proteins. Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody is specific for DNP.
Immunogen
DNP-KLH conjugate
Application
Mouse monoclonal anti-DNP-alkaline phosphatase conjugate antibody can be used for ELISA (1:25,000) and dot blot (1:180,000) assays.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.
The antibody may be used for experimental induction of controlled allergic reactions or as a source of mouse IgE.To induce systemic anaphylaxis, mice were injected with 3 μg of anti-DNP mouse IgE and then given by IV 500 μg of DNP-BSA twenty hours later. To induce local anaphylaxis, mice were injected intradermally with 10ng of anti-DNP mouse IgE and then challenged with an i.v. injection of 500 μg DNP-BSA.
Physical form
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% BSA, and 50% glycerol and 15 mM Sodium azide
Analysis Note
Monoclonal Anti-DNP is tested to ensure minimal reactivity against bovine serum albumin.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Kaouthar Louati et al.
ACS omega, 8(32), 29812-29835 (2023-08-21)
Pesticides are widely used, resulting in continuing human exposure with potential health impacts. Some exposures related to agricultural works have been associated with neurological disorders. Since the 2000s, the hypothesis of the role of pesticides in the occurrence of central
Urszula Krasuska et al.
Frontiers in plant science, 7, 840-840 (2016-07-06)
Canavanine (CAN), a structural analog of arginine (Arg), is used as a selective inhibitor of inducible NOS in mammals. CAN is incorporated into proteins' structure in the place of Arg, leading to the formation of aberrant compounds. This non-protein amino
B Jasani et al.
Journal of immunological methods, 150(1-2), 193-198 (1992-06-24)
Over the past 10 years an immunoperoxidase method using dinitrophenyl (DNP) hapten-labelled primary or secondary probes has been devised. Its widely successful application in research and diagnostic work has depended upon the development of certain key reagents. These include a
Katarzyna Ciacka et al.
Antioxidants (Basel, Switzerland), 11(1) (2022-01-22)
Seed ageing is associated with a high concentration of reactive oxygen species (ROS). Apple (Malus domestica Borkh.) seeds belong to the orthodox type. Due to a deep dormancy, they may be stored in dry condition at 5 °C for a
Sandro Capellmann et al.
Frontiers in immunology, 14, 1154416-1154416 (2023-04-18)
Mast cells (MCs) are immune cells of the myeloid lineage distributed in tissues throughout the body. Phenotypically, they are a heterogeneous group characterized by different protease repertoires stored in secretory granules and differential presence of receptors. To adequately address aspects
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service