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Key Documents

R0600000

Riboflavin

European Pharmacopoeia (EP) Reference Standard

Synonym(s):

(−)-Riboflavin, Lactoflavin, Vitamin B2, Vitamin G

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About This Item

Empirical Formula (Hill Notation):
C17H20N4O6
CAS Number:
Molecular Weight:
376.36
Beilstein:
97825
MDL number:
UNSPSC Code:
41116107
PubChem Substance ID:
NACRES:
NA.24

biological source

fermentation/recombinant (Bacillus subtilis)

grade

pharmaceutical primary standard

Agency

EP

API family

riboflavin

form

solid

manufacturer/tradename

EDQM

technique(s)

gas chromatography (GC): suitable
liquid chromatography (LC): suitable

mp

290 °C (dec.) (lit.)

application(s)

pharmaceutical (small molecule)

format

neat

storage temp.

2-8°C

SMILES string

CC1=C(C)C=C(N(C[C@H](O)[C@@H]([C@H](O)CO)O)C(C2=N3)=NC(NC2=O)=O)C3=C1

InChI

1S/C17H20N4O6/c1-7-3-9-10(4-8(7)2)21(5-11(23)14(25)12(24)6-22)15-13(18-9)16(26)20-17(27)19-15/h3-4,11-12,14,22-25H,5-6H2,1-2H3,(H,20,26,27)/t11-,12+,14-/m0/s1

InChI key

AUNGANRZJHBGPY-SCRDCRAPSA-N

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General description

This product is provided as delivered and specified by the issuing Pharmacopoeia. All information provided in support of this product, including SDS and any product information leaflets have been developed and issued under the Authority of the Issuing Pharmacopoeia. For further information and support please go to the website of the issuing Pharmacopoeia.
Riboflavin is a type of vitamin B that plays a vital role in human nutrition and growth. It finds potential applications in biochemical redox reactions and is present in several foodstuffs, energy drinks, and vitamin formulations.

Application

Riboflavin may be used as an EP reference standard for the determination of the analyte in pharmaceutical formulations by various chromatography techniques.

Packaging

The product is delivered as supplied by the issuing Pharmacopoeia. For the current unit quantity, please visit the EDQM reference substance catalogue.

Other Notes

Sales restrictions may apply.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Simultaneous determination of niacinamide, pyridoxine, riboflavin, and thiamine in multivitamin products by high-pressure liquid chromatography
Walker MC, et al.
Journal of Pharmaceutical Sciences, 70(1), 99-101 (1981)
Simultaneous determination of nicotinamide, pyridoxine hydrochloride, thiamine mononitrate and riboflavin in multivitamin with minerals tablets by reversed-phase ion-pair high performance liquid chromatography
Li K
Biomedical Chromatography, 16(8), 504-507 (2002)
Riboflavin
European Pharmacopoeia Commission and European Directorate for the Quality of Medicines & Healthcare
European pharmacopoeia, 5(2), 3484-3485 (2008)
Differential pulse polarographic method development and validation of riboflavin in pharmaceutical formulation
Kashid LM and Patil SV
International Journal of Scientific Research, 5(2), 325-334 (2015)
Simultaneous determination of riboflavin phosphate and other ingredients in a multivitamin pharmaceutical preparation by on-line automated LC coupled with pre-column immobilized enzyme reactor
Ono M, et al.
Journal of Pharmaceutical and Biomedical Analysis, 29(1-2), 325-334 (2002)

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