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O1875

Sigma-Aldrich

Orcinol monohydrate

≥99% purity (HPLC), powder

Synonym(s):

3,5-Dihydroxytoluene, 5-Methylresorcinol

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About This Item

Linear Formula:
CH3C6H3-1,3-(OH)2 · H2O
CAS Number:
6153-39-5
Molecular Weight:
142.15
Beilstein:
1071903
EC Number:
207-984-2
MDL number:
MFCD00149092
UNSPSC Code:
12171500
PubChem Substance ID:
24897953
NACRES:
NA.47

product name

Orcinol monohydrate, colorimetric detection reagent

biological source

synthetic

Quality Level

200

Assay

≥99% (HPLC)

form

powder

technique(s)

titration: suitable

color

white to light brown

bp

290 °C (lit.)

mp

56-58 °C (lit.)

solubility

H2O: 50 mg/mL

density

1.290 g/cm3

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

O.Cc1cc(O)cc(O)c1

InChI

1S/C7H8O2.H2O/c1-5-2-6(8)4-7(9)3-5;/h2-4,8-9H,1H3;1H2

InChI key

NBKPNAMTHBIMLA-UHFFFAOYSA-N

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General description

Orcinol is a small-molecule natural phenol involved in the production of a dye called orcein used for the staining of cells and chromosomes. Orcinol monohydrate is usually used to prepare a colorimetric reagent known as Bial′s reagent for the determination of RNA, DNA, and hexoses.

Application

Orcinol monohydrate has been used to prepare Bial′s reagent for Bial′s orcinol assay for pentose sugars. It is also used in the preparation of orcinol solution suitable for staining TLC plates and, measuring rhamnolipids in orcinol dye assays. Orcinol monohydrate has been used for the RNA assay.



Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302,H315,H319,H335

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000388065
0000388064
0000385601
0000326628
0000323387

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Marjan De Mey et al.
Analytical biochemistry, 353(2), 198-203 (2006-03-21)
Recent developments in cellular and molecular biology require the accurate quantification of DNA and RNA in large numbers of samples at a sensitivity that enables determination on small quantities. In this study, five current methods for nucleic acid quantification were
A modified orcinol reaction for RNA determination.
M Kamali et al.
Clinical chemistry, 15(5), 390-392 (1969-05-01)
Rapid colorimetric assays to qualitatively distinguish RNA and DNA in biomolecular samples.
Patterson Jennifer and Cameron Mura
Journal of Visualized Experiments (2013)
A simultaneous estimation method of DNA and RNA by the orcinol reaction and a study on the reaction mechanism.
Y Endo
Journal of biochemistry, 67(5), 629-633 (1970-05-01)
Lipopolysaccharide binding protein expression in primary human hepatocytes and HepG2 hepatoma cells.
Grube BJ
The Journal of Biological Chemistry, 269, 8477-8477 (1994)
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