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N5514

Sigma-Aldrich

Nitro Blue Tetrazolium

tablet

Synonym(s):

Nitrotetrazolium Blue Chloride

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About This Item

CAS Number:
298-83-9
MDL number:
MFCD00012159
UNSPSC Code:
12171500
PubChem Substance ID:
24897739
NACRES:
NA.47

form

tablet

Quality Level

200

color

yellow

pH

4.5-5.5

solubility

soluble, clear, yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8

InChI

1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2

InChI key

FSVCQIDHPKZJSO-UHFFFAOYSA-L

Related Categories

General description

Nitro Blue Tetrazolium is a tetrazole compound with two tetrazole rings.

Application

Nitro Blue Tetrazolium has been used:
  • to measure superoxide radicals
  • in lactate dehydrogenase quantification assay
  • for blot development in western blotting analysis
  • in NADPH-d histochemistry
  • as a reagent to analyse the properties of murine intestinal mucins by electrophoresis and histology
  • in immunohistochemistry

Quantity

Contains 10 mg substrate per tablet.

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H302,H315,H318,H335

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000379095
0000342972
0000379095
0000342972
SLCP6317

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Salmonella Serovars and Vaccination Effect on the Immune Responses of Male and Female Layers
Chen Kl, et al.
The journal of poultry science, 53(4), 318-328 (2016)
Nitrotetrazolium blue chloride as a novel corrosion inhibitor of steel in sulfuric acid solution
Deng S, et al
Corrosion Science, 52(11), 3840-3846 (2010)
Analyzing the Properties of Murine Intestinal Mucins by Electrophoresis and Histology
Wang R and Hasnain SZ
PLoS Pathogens (2017)
Night light-adaptation strategies for photosynthetic apparatus in yellow-poplar (Liriodendron tulipifera L.) exposed to artificial night lighting
Kwak MJ, et al.
Forests, 9(2), 74-74 (2018)
Luttmann W
Immunology, The Experimenter Series (2006)
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Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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