B8806
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester
1 mg/mL in DMSO
Synonym(s):
BCECF-AM solution, Spiro(isobenzofuran-1(3H),9′-(9H)xanthene)-2′,7′-dipropanoic acid
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description
Mixture of six isomeric compounds
Quality Level
form
liquid
concentration
1 mg/mL in DMSO
color
colorless to yellow
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
SMILES string
CC(=O)OCOC(=O)CCc1cc2c(Oc3cc(OCOC(C)=O)c(CCC(=O)OCOC(C)=O)cc3C24OC(=O)c5cc(ccc45)C(=O)OCOC(C)=O)cc1OCOC(C)=O
Application
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester has been used for the estimation of vacuolar pH and intracellular pH.
Biochem/physiol Actions
An intracellular fluorescent pH indicator.
Hydrolyzed to BCECF by cytosolic esterases.
Physical form
Solution in 0.1% BCECF AM Ester and 99.9% DMSO
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
185.0 °F
Flash Point(C)
85 °C
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