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222550

Sigma-Aldrich

Nile Blue chloride

Dye content 85 %

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About This Item

Empirical Formula (Hill Notation):
C20H20ClN3O
CAS Number:
2381-85-3
Molecular Weight:
353.85
Colour Index Number:
51180
Beilstein:
3923952
MDL number:
MFCD00011936
UNSPSC Code:
12171500
PubChem Substance ID:
24853342
NACRES:
NA.47

form

powder

Quality Level

200

composition

Dye content, 85%

solubility

water: 1 mg/mL, opaque, blue to very deep blue

λmax

638 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34

InChI

1S/C20H19N3O.ClH/c1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;/h5-12,21H,3-4H2,1-2H3;1H

InChI key

SHXOKQKTZJXHHR-UHFFFAOYSA-N

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Application

Nile blue chloride has been used to stain non-aqueous phase in fluorescence microscopy studies.

Biochem/physiol Actions

Nile blue chloride is a green colored powder and is a member of oxazine group of dyes. It changes its color from blue (pH 9.4) to purple-red (11.0). Nile blue chloride has been used to detect DNA and lipids. In addition, it also helps to identify bacterial growth. Nile blue chloride also facilitates diagnosis of disease linked with amyloid accumulation.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBQ1689
SHBN8805
SHBM3768
14527HA
03126CB

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Effect of ?-casein on surface activity of Quillaja bark saponin at fluid/fluid interfaces
Wojciechowski K
Food Hydrocolloids, 34, 208-216 (2014)
R. W. Sabnis
Handbook of Acid-Base Indicators (2007)
S S Mortazavi et al.
Biological trace element research, 144(1-3), 1430-1436 (2011-07-07)
A simple and sensitive kinetic-spectrophotometric method is developed for the determination of trace amounts of iodine in blood serum samples based on its catalytic effect on the oxidation of Nile Blue A by potassium bromate in sulfuric acid medium and
M Oshiki et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 64(3), 747-753 (2011-11-22)
The present study was conducted (1) to develop a rapid quantification method of polyhydroxyalkanoates (PHA) concentration in activated sludge by Nile blue A staining and fluorescence measurement and (2) to perform on-line monitoring of PHA concentrations in activated sludge. Activated
Pinkee Phukon et al.
Colloids and surfaces. B, Biointerfaces, 92, 30-34 (2011-12-14)
Polyhydroxyalkanoates (PHAs) are naturally occurring polyesters synthesized by bacteria for carbon and energy storage and it has commercial potential as bioplastic. The bacterial species Bacillus circulans MTCC 8167, isolated from crude oil contaminated soil, can efficiently produce medium chain length
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