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222461

Sigma-Aldrich

Murexide

ACS reagent

Synonym(s):

5,5′-Nitrilodibarbituric acid monoammonium salt, Ammonium purpurate

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About This Item

Empirical Formula (Hill Notation):
C8H8N6O6
CAS Number:
3051-09-0
Molecular Weight:
284.19
Colour Index Number:
56085
Beilstein:
3831445
EC Number:
221-266-6
MDL number:
MFCD00012777
UNSPSC Code:
12171500
PubChem Substance ID:
24853333
NACRES:
NA.47

grade

ACS reagent

Quality Level

200

form

powder

technique(s)

titration: suitable

mp

>300 °C (lit.)

λmax

525 nm

suitability

passes test for complexometric indicator

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

N.OC1=C(\N=C2\C(=O)NC(=O)NC2=O)C(=O)NC(=O)N1

InChI

1S/C8H5N5O6.H3N/c14-3-1(4(15)11-7(18)10-3)9-2-5(16)12-8(19)13-6(2)17;/h(H2,10,11,14,15,18)(H3,12,13,16,17,19);1H3

InChI key

LJYRLGOJYKPILZ-UHFFFAOYSA-N

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General description

Murexide is the ammonium salt of purpuric acid that appears as a slightly reddish-purple powder and is soluble in water. It is mainly used as an indicator for complexometric titrations to measure water hardness and as a colorant/pigment in the dye industry.

Application

Murexide is used for the histochemical staining of calcium in the skeleton. It is suitable for staining the hemijaws for dental caries scoring.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM1568
BCCL1516
BCCH6887
BCCF9241
BCCF0688

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Muberra Andac et al.
Journal of fluorescence, 17(4), 401-405 (2007-04-26)
A highly sensitive flow-injection spectrofluorimetric method is presented for the rapid and simple determination of Hg (II) in environmental and pharmaceutical samples. Murexide (ammonium purpurate) was used as the fluorescence reagent in the carrier stream. An emission peak of murexide
P C Pape et al.
The Journal of general physiology, 102(2), 295-332 (1993-08-01)
Cut fibers (striation spacing, 3.6-4.2 microns) were mounted in a double Vaseline-gap chamber and studied at 14-15 degrees C. One or both of the Ca indicators fura-2 and purpurate-3,3' diacetic acid (PDAA) were introduced into the optical recording site by
Indalecio A Peñacoba et al.
Journal of colloid and interface science, 352(2), 465-469 (2010-10-01)
The decomposition reaction of the purple dye murexide in acidic media is used as a probe indicator for protons in nonionic microemulsions. The reaction kinetics primarily rely on the proton concentration and permit assessment of the proton activity in the
D W Hilgemann
The Journal of general physiology, 87(5), 707-735 (1986-05-01)
Extracellular calcium transients were resolved within the time course of single contraction cycles in rabbit left atrium using tetramethylmurexide (2 mM) as the calcium-sensitive dye (150-250 microM total calcium, 80-150 microM free calcium). Net extracellular calcium depletion began within 2-4
D S Jong et al.
The Journal of general physiology, 102(2), 333-370 (1993-08-01)
Cut fibers from Rana temporaria and Rana pipiens (striation spacing, 3.9-4.2 microns) were mounted in a double Vaseline-gap chamber and studied at 14 degrees C. The Ca indicator purpurate-3,3' diacetic acid (PDAA) was introduced into the end pools and allowed
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