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ENDOLYSS-RO

Roche

Endoproteinase Lys-C Sequencing Grade

from Lysobacter enzymogenes

Synonym(s):

lys-c, lysyl endopeptidase, protease

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About This Item

Enzyme Commission number:
3.4.21.50 (BRENDA, IUBMB)
UNSPSC Code:
12352204

biological source

bacterial (Lysobacter enzymogenes)

grade

protein sequencing grade

form

lyophilized

specific activity

≥200 units/mg protein (at 25 °C, with Chromozym PL)

mol wt

33 kDa by SDS-PAGE (reducing)

purified by

electrophoresis

packaging

pkg of 3 × 5 μg (11047825001)
pkg of 5 μg (11420429001)

manufacturer/tradename

Roche

storage condition

(Keep container tightly closed in a dry and well-ventilated place.)

technique(s)

activity assay: suitable

color

white

optimum pH

8.5-8.8

pH range

5-12

solubility

water: soluble

suitability

suitable for enzyme test

UniProt accession no.

Q7M135

storage temp.

2-8°C

Related Categories

General description

Endoproteinase Lys-C Sequencing Grade is isolated from Lysobacter enzymogenes as a highly purified and specific protease. Endoproteinase Lys-C from Lysobacter enzymogenes is a member of the peptidase S1 family. It is mostly used for the initial fragmentation of polypeptide chains in protein sequence analysis.

Specificity

This enzyme is a serine protease that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of Lys (in Tris-HCl buffer, pH 7.0 to 9.0).
Heat inactivation: Enzyme can be denatured using heat (5 min at 100 °C) or by TCA.

Application

Use Endoproteinase Lys-C Sequencing Grade  for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.

Preparation Note

Storage conditions (working solution): +2 to +8 °C

Reconstitution

Lyophilized Endoproteinase Lys-C sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 50 mM Hepes, pH 8.0, 10 mM EDTA and 5 mg/ml raffinose. To avoid autolysis, the incubation temperature should not exceed 37 °C.
Stable in 4 M urea.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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