11767291910
Roche
TUNEL Label Mix
sufficient for 30 tests, pkg of 3 × 550 μL
Synonym(s):
transferase dUTP nick end labeling, tunel
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About This Item
UNSPSC Code:
41105500
Recommended Products
form
solution
Quality Level
usage
sufficient for 30 tests
packaging
pkg of 3 × 550 μL
manufacturer/tradename
Roche
color
colorless
solubility
water: miscible
storage temp.
−20°C
General description
The nucleotide-labeling mix (TUNEL Label) contains fluorescein-dUTP and -dNTPs, both needed to perform the TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) reaction for the detection of apoptosis in situ. The nucleotide-labeling mix is used in combination with the TUNEL enzyme to prepare a TUNEL reaction mixture. This reaction mixture is used to label DNA-strand breaks for detecting and quantifying apoptotic cell death at a single-cell level in cells and tissues.
Application
TUNEL Label Mix has been used for the determination of apoptosis using TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay and DNA nick-end labeling by the TUNEL method.
The nucleotide-labeling mix (TUNEL Label) contains fluorescein-dUTP and -dNTPs, both needed to perform the TUNEL reaction for the detection of apoptosis in situ. The nucleotide-labeling mix is used in combination with the TUNEL Enzyme to prepare a TUNEL reaction mixture. This reaction mixture is used to label DNA-strand breaks for detecting and quantifying apoptotic cell death at a single-cell level in cells and tissues.
Preparation Note
Working solution: TUNEL Label is used in combination with the TUNEL Enzyme to prepare the TUNEL reaction mixture.
For one test: Mix 45 μl TUNEL Label with 5 μl TUNEL Enzyme prior to use. For negative control use 50 μl/test TUNEL Label only.
Storage conditions (working solution): Note: The TUNEL reaction mixture (45 μl TUNEL Label with 5 μl TUNEL Enzyme for 1 test) should be prepared just before use, and should not be stored. Keep the TUNEL reaction mixture on ice until use.
For one test: Mix 45 μl TUNEL Label with 5 μl TUNEL Enzyme prior to use. For negative control use 50 μl/test TUNEL Label only.
Storage conditions (working solution): Note: The TUNEL reaction mixture (45 μl TUNEL Label with 5 μl TUNEL Enzyme for 1 test) should be prepared just before use, and should not be stored. Keep the TUNEL reaction mixture on ice until use.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Carc. 1B Inhalation
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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