11175041910
Roche
DIG Nucleic Acid Detection Kit
sufficient for 40 blots (10 cm x 10 cm each), kit of 1 (5 components), suitable for hybridization, suitable for Northern blotting
Synonym(s):
DIG system
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About This Item
UNSPSC Code:
41116134
Recommended Products
usage
sufficient for 40 blots (10 cm x 10 cm each)
Quality Level
packaging
kit of 1 (5 components)
manufacturer/tradename
Roche
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
greener alternative category
, Aligned
storage temp.
−20°C
General description
DIG Nucleic Acid Detection Kit is used for detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP (alkaline phosphatase) antibody conjugate and the color substrates NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate).
DIG Nucleic Acid Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP (alkaline phosphatase) antibody conjugate and the color substrates NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate).
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application
DIG nucleic acid detection kit has been used in the confirmation of genetic manipulation.
The DIG Nucleic Acid Detection Kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:
- Southern blots
- Northern blots
- Other nucleic acid blotting applications
- In situ hybridization applications
The kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:
- Southern blots
- Northern blots
- Other nucleic acid blotting applications
- In situ hybridization applications
Features and Benefits
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Packaging
1 kit containing 5 components.
Principle
DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP antibody conjugate and the color substrates NBT and BCIP.
Preparation Note
Working concentration: Flow cytometry: 2 μg/ml (0.2 μg/100 μl/106 cells); Immunohistocytochemistry: 6 μg/ml
Working concentration of conjugate depends on application and substrate.
Storage conditions (working solution):
Working concentration of conjugate depends on application and substrate.
Storage conditions (working solution):
- Anti-Digoxigenin-AP Conjugate (vial 3): 2 to 8 °C, it is stable for 12 months at this temperature
- NBT/BCIP (vial 4): 2 to 8 °C, stable
Note: During shipment of the kit on dry ice, a precipitate may occur which is dissolved by briefly warming to 37 °C - The blocking reagent (vial 5) is stable for 36 months and can be stored dry at 4 to 8 °C.
- Autoclaved stock solution can be stored for several days to a week either unopened at 15 to 25 °C or at 4 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.
Kit Components Only
Product No.
Description
- DIG-labeled Control DNA 5 µg/ml
- DNA Dilution Buffer, [50 μg/ml] fish sperm DNA
- Anti-Digoxigenin-AP Conjugate antibody 750 U/ml
- NBT/BCIP, 50x stock solution 50x concentrated
- Blocking Reagent
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
does not flashNot applicable
Flash Point(C)
does not flashNot applicable
Certificates of Analysis (COA)
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Articles
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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