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11089153001

Roche

Streptavidin-POD conjugate

liquid, pkg of 1 mL

Synonym(s):

streptavidin

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About This Item

UNSPSC Code:
12352200

form

liquid

Quality Level

packaging

pkg of 1 mL

manufacturer/tradename

Roche

storage temp.

-10 to -25°C

General description

Streptavidin is covalently coupled to horseradish peroxidase. The conjugate is used for detection of biotin-labelled substances.

Application

Streptavidin-POD has been used for:
  • ELISA
  • In situ hybridization
  • Western blot
It is also used for immunohistochemistry.

Unit Definition

One unit Streptavidin-POD, corresponding to one unit peroxidase, will oxidize 1 μmol of guaiacol tetramer in one minute at +25 °C and pH 7.0.

Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).

Physical form

Liquid, stabilized

Preparation Note

Working concentration: 5 - 500 mU/ml (i.e., stock solution can be diluted).Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: 50 to 200 mU/ml
  • Immunohistocytochemistry: 250 to 1000 mU/ml
  • Western blot: 200 to 500 mU/ml

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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