OP07
Anti-v-Src (Ab-1) Mouse mAb (327)
liquid, clone 327, Calbiochem®
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
327, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
mouse, human, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... SRC(6714)
General description
Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Recognizes the ~60 kDa Src protein.
Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.
This Anti-v-Src (Ab-1) Mouse mAb (327) is validated for use in Immunoblotting, Immunoprecipitation, Frozen Sections, Immunofluorescence, Paraffin Sections for the detection of v-Src (Ab-1).
Immunogen
Epitope: within the SH3 domain of v-Src and c-Src
purified v-Src
Application
Immunoblotting (2.5 µg/ml, see application references)
Immunoprecipitation (1 µg antibody or use Cat. No. OP07A; see application references)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Paraffin Sections (not recommended)
Immunoprecipitation (1 µg antibody or use Cat. No. OP07A; see application references)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Paraffin Sections (not recommended)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
Analysis Note
Positive Control
NIH/3T3 cells
NIH/3T3 cells
Other Notes
Courtneidge, S.A. and Heber, A. 1987. Cell50, 1031.
Cartwright, C.A., et al. 1985. Mol. Cell. Biol.5,, 2647.
Courtneidge, S.A. 1985. EMBO J.4, 1471.
Whitman, M., et al. 1985. Nature315, 239
Bolen, J.B., et al. 1984. Cell38, 767.
Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci.77, 1311.
Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci.76, 3159.
Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci.75, 2021.
Collett, M.S., et al. 1978. Cell15, 1363.
Cartwright, C.A., et al. 1985. Mol. Cell. Biol.5,, 2647.
Courtneidge, S.A. 1985. EMBO J.4, 1471.
Whitman, M., et al. 1985. Nature315, 239
Bolen, J.B., et al. 1984. Cell38, 767.
Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci.77, 1311.
Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci.76, 3159.
Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci.75, 2021.
Collett, M.S., et al. 1978. Cell15, 1363.
pp60src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active src kinase (see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Lisa M Mehlmann et al.
Reproduction (Cambridge, England), 129(5), 557-564 (2005-04-28)
SRC family kinases (SFKs) function in initiating Ca2+ release at fertilization in several species in the vertebrate evolutionary line, but whether they play a similar role in mammalian fertilization has been uncertain. We investigated this question by first determining which
Soshi Noshita et al.
Journal of cell science, 136(15) (2023-07-13)
The non-receptor tyrosine kinase SRC is overexpressed and/or hyperactivated in various human cancers, and facilitates cancer progression by promoting invasion and metastasis. However, the mechanisms underlying SRC upregulation are poorly understood. In this study, we demonstrate that transforming growth factor-β
L Dulabon et al.
Neuron, 27(1), 33-44 (2000-08-12)
Mice that are mutant for Reelin or Dab1, or doubly mutant for the VLDL receptor (VLDLR) and ApoE receptor 2 (ApoER2), show disorders of cerebral cortical lamination. How Reelin and its receptors regulate laminar organization of cerebral cortex is unknown.
Daiju Kitagawa et al.
The Journal of biological chemistry, 277(1), 366-371 (2001-11-06)
Ultraviolet (UV) irradiation stimulates stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK), which is a member of the mitogen-activated protein kinase (MAPK) superfamily and implicated in stress-induced apoptosis. UV also induces the activation of another MAPK member, extracellular signal-regulated kinase (ERK), which
Xiaoming Ju et al.
Cancer research, 76(22), 6723-6734 (2016-11-05)
Proteomic analysis of castration-resistant prostate cancer demonstrated the enrichment of Src tyrosine kinase activity in approximately 90% of patients. Src is known to induce cyclin D1, and a cyclin D1-regulated gene expression module predicts poor outcome in human prostate cancer.
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