MAB2514
Anti-Integrin α5β1 Antibody, clone BMB5
ascites fluid, clone BMB5, Chemicon®
Synonym(s):
VLA-5
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
BMB5, monoclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG3
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... ITGA5(3678)
Specificity
Antibody MAB2514 was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.
Immunogen
The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.
Application
Inhibition of cell adhesion to fibronectin: 10 μg/mL
Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMB5 alone may not be complete.
Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μg
Flow cytometry: 10μg/mL
Western blot (non-denaturing gel)
Optimal working dilutions must be determined by end user.
Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMB5 alone may not be complete.
Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μg
Flow cytometry: 10μg/mL
Western blot (non-denaturing gel)
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
This Anti-Integrin α5β1 Antibody, clone BMB5 is validated for use in FC, IP, WB, IH, FUNC for the detection of Integrin α5β1.
Physical form
Liquid, containing no preservatives.
Storage and Stability
Maintain frozen at -20°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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