ABT174
Anti-LGN Antibody
serum, from rabbit
Synonym(s):
G-protein-signaling modulator 2, Mosaic protein LGN, LGN
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Gpsm2(76123)
General description
LGN is also known as G-protein-signaling modulator 2 (GPSM2). LGN has an important role in cellular structure and spindle pole orientation. LGN also stabilizes the apical complex during neuroblast divisions. LGN defects lead to Chudley-McCullough syndrome as well as a multiple micronuclei phenotype due to chromosomal mis-segregation and cell division errors.
Specificity
Other Homologies: Human (85% sequence homology), Chicken (75% sequence homology).
Immunogen
GST-tagged recombinant protein corresponding to mouse LGN.
Application
Anti-LGN Antibody is a highly specific rabbit polyclonal antibody, that targets LGN & has been tested in western blotting.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LGN in mouse E16 brain tissue lysate.
Quality
Evaluated by Western Blotting in rat E16 heart tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LGN in rat E16 heart tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LGN in rat E16 heart tissue lysate.
Target description
~77 kDa observed. Uncharacterized bands may be observed at ~30 kDa, ~60 kDa, and ~70 kDa in some cell lysates.
Physical form
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Heart tissue lysate
Heart tissue lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ouranio Anastasiou et al.
Science signaling, 13(620) (2020-02-27)
Correct selection of the cell division axis is important for cell differentiation, tissue and organ morphogenesis, and homeostasis. Both integrins, which mediate interactions with extracellular matrix (ECM) components such as fibronectin, and cadherins, which mediate interactions between cells, are implicated
Carlos P Descovich et al.
eLife, 12 (2023-04-06)
Oriented cell divisions balance self-renewal and differentiation in stratified epithelia such as the skin epidermis. During peak epidermal stratification, the distribution of division angles among basal keratinocyte progenitors is bimodal, with planar and perpendicular divisions driving symmetric and asymmetric daughter
Kanako Chishiki et al.
Genes to cells : devoted to molecular & cellular mechanisms, 22(3), 293-309 (2017-02-12)
Correct cyst morphogenesis of epithelial cells requires apical-basal polarization, which is partly regulated by mitotic spindle orientation, a process dependent on the heterotrimeric G protein subunit Gαi and its binding protein LGN. Here, we show that in three-dimensional culture of
Par3-mInsc and G?i3 cooperate to promote oriented epidermal cell divisions through LGN.
Williams, SE; Ratliff, LA; Postiglione, MP; Knoblich, JA; Fuchs, E
Nature Cell Biology null
Kendall J Lough et al.
eLife, 8 (2019-12-14)
During organogenesis, precise control of spindle orientation balances proliferation and differentiation. In the developing murine epidermis, planar and perpendicular divisions yield symmetric and asymmetric fate outcomes, respectively. Classically, division axis specification involves centrosome migration and spindle rotation, events occurring early
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