ABN90P
Anti-NeuN purified Antibody
from guinea pig, purified by affinity chromatography
Synonym(s):
NEUronal Nuclei, clone A60, NeuN
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
guinea pig
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat, mouse
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RBFOX3(146713)
mouse ... Rbfox3(52897)
rat ... Rbfox3(287847)
General description
ABN90P is a Guinea Pig polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are some examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
Specificity
This antibody recognizes the N-terminus of NeuN.
Immunogen
Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.
Application
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NeuN in mouse frontal cortex tissue.
Immunocytochemistry Analysis: A 1:1,000-2,000 dilution from a representative lot detected NeuN in rat E18 cortex cells.
Immunocytochemistry Analysis: A 1:1,000-2,000 dilution from a representative lot detected NeuN in rat E18 cortex cells.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
This Anti-NeuN purified Antibody is validated for use in Western Blotting, ICC, IHC(P) for the detection of NeuN purified.
Quality
Evaluated by Western Blot in mouse E16 brain tissue lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected NeuN on 10 µg of mouse E16 brain tissue lysate.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected NeuN on 10 µg of mouse E16 brain tissue lysate.
Target description
~48 kDa observed
Physical form
Antigen Affinity Purified
Purified guinea pig polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse E16 brain tissue lysate
Mouse E16 brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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