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Key Documents

AB5802

Sigma-Aldrich

Anti-Dopamine Transporter Antibody, extracellular Loop 2

Chemicon®, from rabbit

Synonym(s):

Anti-DAT

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, monkey

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SLC6A3(6531)

Specificity

Dopamine Transporter, Extracellular loop 2 (EL2).

Immunogen

Epitope: extracellular Loop 2
Synthetic peptide from the EL2 region of human dopamine transporter.

Application

Anti-Dopamine Transporter Antibody, extracellular Loop 2 is an antibody against Dopamine Transporter for use in IH & WB.
Immunoblotting: 1:500-1:1,000

Immunohistochemistry using formaldehyde-fixed brain sections: 1:500-1:1,000

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Physical form

Affinity purified immunoglobulin. Each vial contains ~33 μg of affinity-purified antibody in 100 μL of 10 mM HEPES (pH 7.5), 150 mM NaCl with 100 μg/mL BSA and 50% glycerol.

Storage and Stability

Maintain at -20 to -80°C in undiluted aliquots for up to six months. Store in a NON-self-defrosting freezer. Avoid repeated freeze-thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adithya Gopinath et al.
NPJ Parkinson's disease, 8(1), 72-72 (2022-06-08)
Parkinson's disease (PD) is marked by a loss of dopamine neurons, decreased dopamine transporter (DAT) and tyrosine hydroxylase (TH) expression. However, this validation approach cannot be used for diagnostic, drug effectiveness or investigational purposes in human patients because midbrain tissue
Physical and functional interaction between the dopamine transporter and the synaptic vesicle protein synaptogyrin-3.
Ega?a, LA; Cuevas, RA; Baust, TB; Parra, LA; Leak, RK; Hochendoner, S; Pe?a, K; Quiroz et al.
The Journal of Neuroscience null
Etienne Cartier et al.
Frontiers in cellular neuroscience, 13, 35-35 (2019-03-05)
The dopamine transporter (DAT) is a plasma membrane protein responsible for the uptake of released dopamine back to the presynaptic terminal and ending dopamine neurotransmission. The DAT is the molecular target for cocaine and amphetamine as well as a number
Abeer Dagra et al.
NPJ Parkinson's disease, 7(1), 76-76 (2021-08-20)
Pathophysiological damages and loss of function of dopamine neurons precede their demise and contribute to the early phases of Parkinson's disease. The presence of aberrant intracellular pathological inclusions of the protein α-synuclein within ventral midbrain dopaminergic neurons is one of

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