AB1607
Anti-Phosphothreonine Antibody
Chemicon®, from rabbit
Synonym(s):
Phospho-Threonine Antibody
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
shipped in
wet ice
target post-translational modification
phosphorylation (pThr)
General description
Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr.
Specificity
Anti-phosphothreonine is species independent
By direct competitive ELISA methodology (antigen capture) the specificity of this antibody has been determined to be phosphothreonine. Binding of Ly-pT-Gly-HRP (0.2μg/100μL/well) to an anti-phosphothreonine coated microplate (0.5μg/100μL/well) is inhibited by 95% with the addition of 2-10 μg/mL anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine, or threonine at concentrations of up to 1 mg/mL. Antibody AB1607 recognizes phosphothreonine, peptidyl-phosphothreonine, and threonine-phosphorylated proteins.
Immunogen
Keyhole Limpet Hemocyanin (KLH) – conjugated phosphothreonine conjugates and Phosvitin.
Application
Anti-Phosphothreonine Antibody is an antibody against Phosphothreonine for use in ELISA, IH(P), IP & WB.
ELISA (kinase assay):
A 0.5 μg/mL concentration of a previous lot was used in ELISA.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Immunohistochemistry (paraffin):
Representative image from a previous lot.
Optimal working dilutions must be determined by end user.
A 0.5 μg/mL concentration of a previous lot was used in ELISA.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Immunohistochemistry (paraffin):
Representative image from a previous lot.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Quality
Routinely evaluated by Western Blot on Calyculin/Okadaic Acid treated HEK293 lysate.
Western Blot Analysis: 1:500 dilution of this lot detected PHOSPHOTHREONINE on 10 μg of Calyculin/Okadaic Acid treated HEK293 lysate.
Western Blot Analysis: 1:500 dilution of this lot detected PHOSPHOTHREONINE on 10 μg of Calyculin/Okadaic Acid treated HEK293 lysate.
Physical form
ImmunoAffinity Purified
Purified rabbit polyclonal in PBS buffer, containing 50% Glycerol, 0.01% NaN3.
Storage and Stability
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Analysis Note
Control
Mouse brain lysate.
Mouse brain lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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