688002-M

Y-27632

≥95% (HPLC), liquid, Rho kinase inhibitor, Calbiochem^®

• Synonym(s): InSolution Y-27632 in DMSO, Rho Kinase Inhibitor VI, (R)-(+)- trans-N-(4-Pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide, 2HCl
• Empirical Formula (Hill Notation): C₁₄H₂₁N₃O · 2HCl · xH₂O
• Molecular Weight: 320.26 (anhydrous basis)
• UNSPSC Code: 12352200

Properties

• Product Name: Y-27632 in DMSO, InSolution, ≥95%, 10 mM, reversible and selective inhibitor of Rho-associated protein kinases
• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles; desiccated (hygroscopic); protect from light
• storage temp.: −70°C

Description

General description

A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (K_i = 140 nM for p160^ROCK). Also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP. Exhibits 10- to 50-fold lower affinity for PKC_ε than p160^ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at higher concentrations (~100 µM). Also acts as a potent inhibitor of agonist-induced Ca²⁺ sensitization of myosin phosphorylation and smooth muscle contraction. Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency.

Warning

Toxicity: Irritant (B)

Physical form

A 10 mM (1 mg/296 µl) solution of Y-27632 (Cat. No. 688000)in DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Watanabe, K., et al. 2007. Nature Biotech.25, 681.
Chitaley, K., et al. 2001. Nat. Med.7, 119.
Davies, S.P., et al. 2000. Biochem. J. 351, 95.
Narumiya, S., et al. 2000. Methods Enzymol.325, 273.
Hirose, M., et al. 1998. J. Cell Biol.141, 1625.
Maekawa, M., et al. 1999. Science285, 895.
Uehata, M., et al. 1997. Nature389, 990.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flash Point(C): 87 °C - closed cup - (Dimethylsulfoxide)