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Millipore

Protein A Agarose

Protein A Agarose Beads for the purification of human, mouse & rabbit immunoglobins.

Synonym(s):

Protein A resin

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About This Item

UNSPSC Code:
41116133
eCl@ss:
32160801
NACRES:
NA.56

form

liquid

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

shipped in

wet ice

General description

Protein A affinity adsorbent possessing a high antibody-binding capacity is significantly used for the purification of biopharmaceuticals. Protein A agarose beads contain sufficient covalently-linked Protein A for a binding capacity of 20+/-2 milligrams human IgG per 1 milliliter.

Application

Protein A Agarose has been used in immunoprecipitation. It is suitable for the purification of human, mouse, and rabbit immunoglobulins.

Quality

routinely evaluated by immunoprecipitating phosphotyrosine containing proteins from an EGF-stimulated A431 cell lysate with Anti-Phosphotyrosine antibody (05-321)

Physical form

water containing 0.01% thimerosal

Storage and Stability

1 year at 4°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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D K Fuhrer et al.
The Journal of biological chemistry, 270(42), 24826-24830 (1995-10-20)
Protein tyrosine phosphorylation and thus dephosphorylation are part of the interleukin (IL)-11 response in mouse 3T3-L1 cells. We report here for the first time the involvement and interactions of the SH2-containing protein tyrosine phosphatase Syp in the IL-11 signal transduction
Identification of regions within the four small subunits of human replication factor C required for complex formation and DNA replication
Uhlmann, F., et al
The Journal of Biological Chemistry, 272, 10065-10071 (1997)
Activation of endothelin ETA receptors induces phosphorylation of alpha1b-adrenoreceptors in Rat-1 fibroblasts
Vazquez-Prado, J., et al
The Journal of Biological Chemistry, 272, 27330-27337 (1997)
Pertussis toxin-sensitive G proteins as mediators of the signal transduction pathways activated by cytomegalovirus infection of smooth muscle cells.
Shibutani, T, et al.
The Journal of Clinical Investigation, 100, 2054-2061 (1997)
Michael P Stany et al.
PloS one, 6(7), e21121-e21121 (2011-07-15)
Clear cell ovarian cancer is an epithelial ovarian cancer histotype that is less responsive to chemotherapy and carries poorer prognosis than serous and endometrioid histotypes. Despite this, patients with these tumors are treated in a similar fashion as all other

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