1.52370
Chromolith® WP 300 RP-18 (monolithic) HPLC Columns
L × I.D. 10 cm × 2 mm HPLC Column
Synonym(s):
Chromolith® WP 300 RP-18 100-2 HPLC column
About This Item
Recommended Products
product name
Chromolith® WP 300 RP-18, L × I.D. 10 cm × 2 mm HPLC column
material
PEEK column
Quality Level
Agency
suitable for USP L1
product line
Chromolith®
feature
endcapped: no
packaging
1 ea of
extent of labeling
18% loading
parameter
200 bar pressure
60 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable
column L × I.D.
10 cm × 2 mm
surface area
300 m2/g
matrix
monolithic silica
matrix active group
C18 bonding phase
pore size
2 μm macroporosity
30 mm mesoporosity
operating pH range
2-7.5
separation technique
reversed phase
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General description
The Chromolith® WP 300 RP-18 column gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry incorporated into the Chromolith® WP 300 line improve resolution by increasing efficiency and reducing peak tailing.
Chromolith® WP 300 columns are made of monolithic silica with a characteristic bimodal pore structure.
Chromolith® WP 300 RP-18, 2 mm I.D. columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 30 nm (300 Å), the surface modification is Octadecylsilane. Chromolith® WP 300 RP-18 columns fall under the USP L1 Classification. Chromolith® WP 300 RP-18 columns are fully suitable for LC-MS applications.
The column dimension of 100 mm length and 2 mm I.D. is the best choice for fast separations of Biomolecules on any UHPLC System.
Chromolith® WP 300 RP-18, 100-2 HPLC columns enable:
—Completely bioinert column hardware and high biorecovery
—High-speed separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® WP 300 RP-18, 5-2 guard cartridges (3 pieces) [1.52372] with the Chromolith® Guard Cartridge Holder Bioinert 5 mm x 2 mm [1.52355]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series. [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
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Articles
Compare columns in resolving medium-sized antibody fragments after digestion with DTT or IdeS using Reversed-Phase Chromatography for analysis.
Compare columns in resolving medium-sized antibody fragments after digestion with DTT or IdeS using Reversed-Phase Chromatography for analysis.
Compare columns in resolving medium-sized antibody fragments after digestion with DTT or IdeS using Reversed-Phase Chromatography for analysis.
Compare columns in resolving medium-sized antibody fragments after digestion with DTT or IdeS using Reversed-Phase Chromatography for analysis.
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