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Key Documents

05-636

Sigma-Aldrich

Anti-phospho-Histone H2A.X (Ser139) Antibody, clone JBW301

clone JBW301, Upstate®, from mouse

Synonym(s):

H2AXS139P, Histone H2A.X (phospho S139)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

affinity purified immunoglobulin

clone

JBW301, monoclonal

species reactivity

vertebrates

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NP_002096

UniProt accession no.

P16104

shipped in

wet ice

target post-translational modification

phosphorylation (pSer139)

General description

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Specificity

Anti-phospho-Histone H2A.X (Ser139) Antibody clone JBW301, recognizes Histone H2A.X phosphorylated at Ser139.

Immunogen

peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X

Application

Additional Referenced Applications:
Immunohistochemistry Analysis: A representative lot detected Histone H2A.X (pSer139) in RNF168-WT and RNF 168-SA/SEKI mice lung tissue sections (Paraffin). (Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331).
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.
Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Immunoblot Analysis: 0.05-1 μg/ml of this antibody detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 μM staurosporine (Catalog # 19-123).
Immunocytochemistry: 2 μg/ml of this antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5 μM staurosporine for 4-6 hours.

Target description

17 kDa

Linkage

Replaces: MABE205

Physical form

Format: Purified
Immunoaffinity Purified immunoglobulin in 0.1M Tris-Glycine,pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative.
Protein G Purified

Storage and Stability

Maintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Analysis Note

Control
UV-treated 293 cell extracts, UV-treated HeLa cell extracts or breast cancer tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Incomplete nuclear transformation of human spermatozoa in oligo-astheno-teratospermia: characterization by indirect immunofluorescence of chromatin and thiol status.
Ramos, L; van der Heijden, GW; Derijck, A; Berden, JH; Kremer, JA; van der Vlag, J; de Boer, P
Human Reproduction null
DNA interaction and dual topoisomerase I and II inhibition properties of the anti-tumor drug prodigiosin.
Montaner, B; Castillo-Avila, W; Martinell, M; Ollinger, R; Aymami, J; Giralt, E; Perez-Tomas, R
Toxicological Sciences null
DNA damage response and apoptosis.
Plesca, D; Mazumder, S; Almasan, A
Methods in Enzymology null
Lidia Ruiz et al.
PloS one, 3(9), e3230-e3230 (2008-09-19)
P53 activation can trigger various outcomes, among them reversible growth arrest or cellular senescence. It is a live debate whether these outcomes are influenced by quantitative or qualitative mechanisms. Furthermore, the relative contribution of p53 to Ras-induced senescence is also
Effect of Ku80 deficiency on mutation frequencies and spectra at a LacZ reporter locus in mouse tissues and cells.
Busuttil, RA; Mu?oz, DP; Garcia, AM; Rodier, F; Kim, WH; Suh, Y; Hasty, P; Campisi, J; Vijg, J
Testing null
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