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05-499

Sigma-Aldrich

Anti-Histone H3 Antibody, clone 6.6.2

clone 6.6.2, Upstate®, from mouse

Synonym(s):

H3 histone, family 3A

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6.6.2, monoclonal

species reactivity

vertebrates, human, plant

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... H3F3B(3021)

General description

This monoclonal antibody was generated using an unmodified peptide corresponding to the first 20 amino acids of histone H3 and is specific for an unmodified epitope in that region.

Specificity

This antibody recognizes and is specific for histone H3, Mr 17kDa.
This peptide sequence is identical in a wide range of animal and plant species.

Immunogen

Epitope: a.a. 1-20
KLH conjugated peptide corresponding to amino acids 1-20 [ARTKQTARKSTGGKAPRKQLC] of human histone H3. Clone 6.6.2.

Application

Anti-Histone H3 Antibody, clone 6.6.2 is a Mouse Monoclonal Antibody for detection of Histone H3 also known as H3 histone family 3A & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Routinely evaluated by western blot on acid extracted lysates from HeLa cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected Histone H3 in acid extracted lysates from HeLa cells.

Target description

17 kDa

Physical form

Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG1κ in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide and 30% glycerol.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.

Analysis Note

Control
HeLa cell extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A HIGHLY SELECTIVE OLIGOPEPTIDE BINDING PROTEIN FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS.
Gogliettino M, Balestrieri M, Pocsfalvi G, Fiume I, Natale L, Rossi M, Palmieri G
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Macari, ER; Lowrey, CH
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Phase I pharmacokinetic and pharmacodynamic evaluation of combined valproic acid/doxorubicin treatment in dogs with spontaneous cancer.
Wittenburg LA, Gustafson DL, Thamm DH
Clinical cancer research : an official journal of the American Association for Cancer Research null
Glycosylation and sialylation of macrophage-derived human apolipoprotein E analyzed by SDS-PAGE and mass spectrometry: evidence for a novel site of glycosylation on Ser290.
Lee Y, Kockx M, Raftery MJ, Jessup W, Griffith R, Kritharides L
Molecular and Cellular Proteomics null
Brian D Cherrington et al.
PloS one, 5(7), e11768-e11768 (2010-07-30)
Peptidylarginine Deiminases (PADs) convert arginine residues on substrate proteins to citrulline. Previous reports have documented that PAD2 expression and activity varies across the estrous cycle in the rodent uterus and pituitary gland, however, the expression and function of PAD2 in

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