707066

DCA Deblock (0.36M dichloroacetic acid in toluene)

• Synonym(s): DCA Deblock, DEBLOCK (0.36M dichloroacetic acid in toluene)
• Empirical Formula (Hill Notation): C₂H₂Cl₂O₂
• CAS Number: 79-43-6
• Molecular Weight: 128.94
• MDL number: MFCD00004223
• UNSPSC Code: 12352106
• PubChem Substance ID: 329762686

Properties

• form: liquid
• Quality Level: 100
• concentration: ≤100 ppm in H₂O
• bp: 110-111 °C
• density: 0.884 g/mL at 25 °C
• SMILES string: OC(=O)C(Cl)Cl
• InChI: 1S/C2H2Cl2O2/c3-1(4)2(5)6/h1H,(H,5,6)
• InChI key: JXTHNDFMNIQAHM-UHFFFAOYSA-N

Description

General description

Deblock solution, containing dichloroacetic acid (DCA) in dichloromethane or Toluene, removes the dimethoxytrityl (DMT) protecting the group from the 5′ hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′ hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Application

DCA Deblock (0.36M dichloroacetic acid in toluene) is suitable for the removal of 4,4Adimethoxytrityl (DMTr) protecting groups for the synthesis of antisense oligonucleotides in high yield and purity.

Safety Information

• Pictograms: GHS02,GHS08,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H304,H315,H318,H336,H351,H360FD,H362,H373,H412
• Precautionary Statements: P210 - P263 - P280 - P301 + P310 - P305 + P351 + P338 - P331
• Hazard Classifications: Aquatic Chronic 3 - Asp. Tox. 1 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 2 - Lact. - Repr. 1B - Skin Irrit. 2 - STOT RE 2 Inhalation - STOT SE 3
• Target Organs: Central nervous system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 41.0 °F
• Flash Point(C): 5 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves