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Key Documents

214515

Sigma-Aldrich

Chromotrope FB

Dye content 50 %

Synonym(s):

Acid Red 14, Disodium 4-hydroxy-3-[(4-sulfo-1-naphthalenyl)azo]-1-naphthalenesulfonate, Mordant Blue 79

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About This Item

Empirical Formula (Hill Notation):
C20H12N2Na2O7S2
CAS Number:
Molecular Weight:
502.43
Colour Index Number:
14720
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

composition

Dye content, 50%

mp

275 °C

λmax

383 nm
515 nm (2nd)

ε (extinction coefficient)

≥14500 at 513-519 nm in H2O at 0.03 g/L
≥33000 at 214-220 nm in H2O at 0.03 g/L
≥9000 at 320-326 nm in H2O at 0.03 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1c(cc(c2ccccc12)S([O-])(=O)=O)\N=N\c3ccc(c4ccccc34)S([O-])(=O)=O

InChI

1S/C20H14N2O7S2.2Na/c23-20-15-8-4-3-7-14(15)19(31(27,28)29)11-17(20)22-21-16-9-10-18(30(24,25)26)13-6-2-1-5-12(13)16;;/h1-11,23H,(H,24,25,26)(H,27,28,29);;/q;2*+1/p-2/b22-21+;;

InChI key

YSVBPNGJESBVRM-ZPZFBZIMSA-L

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Application

Chromotrope FB has been used in indirect competitive CLIA (icCLIA) to detect its presence in foods.

Biochem/physiol Actions

Chromotrope FB, also known as acid red 14 (AR14), is a bright orange-red powder. It belongs to the class of monoazides. Chromotrope FB efficiently stains fatty substances and is also used to dye food and medicines.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

>437.0 °F

Flash Point(C)

> 225 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Marcel Locquin, Maurice Langeron
Handbook of Microscopy (2013)
Wayne Luallen
Tying the Classic Salmon Fly: A Modern Approach to Traditional Techniques (1997)
A sensitive chemiluminescent immunoassay to detect Chromotrope FB (Chr FB) in foods
Xu K
Talanta, 164, 341-347 (2017)
Meenakshi Tripathi et al.
Journal of AOAC International, 87(3), 657-663 (2004-08-04)
A simple method has been developed for the extraction, separation, and determination of synthetic colors in ice cream samples. The process involves the breakdown of emulsion by neutral detergents (Triton X-100 and Tween 20) followed by extraction with petroleum ether
E Tragni et al.
Journal of applied toxicology : JAT, 5(5), 273-276 (1985-10-01)
Pregnant rats received 14C-Carmoisine (200 mg kg-1; 25 microCi) by gavage on days 16-19 of gestation. The animals were killed and maternal tissues, amniotic fluid, placentae, foetal membranes and foetuses were analyzed for radioactivity. No evidence for the transplacental transfer

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