SAB4200591

Anti-unmodified-Histone H3 (Lys9) antibody, Mouse monoclonal

clone 9B1-2G6, purified from hybridoma cell culture

• Synonym(s): Anti-HIST1H3A H3FA, Anti-HIST1H3B H3FL, Anti-HIST1H3C H3FC, Anti-HIST1H3D H3FB, Anti-HIST1H3E H3FD, Anti-HIST1H3F H3FI, Anti-HIST1H3G H3FH, Anti-HIST1H3H H3FK, Anti-HIST1H3I H3FF, Anti-HIST1H3J H3FJ, Anti-HIST2H3A, Anti-HIST2H3C H3F2 H3FM, Anti-HIST2H3D, Monoclonal Anti-unmodified-Histone H3 (Lys⁹) antibody produced in mouse
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 9B1-2G6, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~17 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 2-4 μg/mL using histones isolated from human HeLa cells; immunofluorescence: 2-4 μg/mL using human HeLa cells
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... H3C1(8350)

Description

General description

Monoclonal Anti-unmodified-Histone H3 (Lys9 ) (mouse IgG1 isotype) is derived from the hybridoma 9B1-2G6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide. Histone H3 is a core component of histone octamer of nucleosome along with other histones H4, H2A and H2B. Histone H3 comprises lysine and arginine residues in the N-terminal region. Conserved lysine residues in the N-terminal tail domains of histone H3 comprise theLys4, Lys9 and Lys27, which are the preferred sites of methylation.

Specificity

Monoclonal Anti-unmodified-Histone H3 (Lys9 ) recognizes human unmodified-Histone H3 (Lys9 ) (nontested in other species). This antibody also binds to peptides containing mono-, and di-methyl-Lys4 and acetyl-Lys14 of Histone H3. It does not bind to peptides containing mono-, di-, and tri-methyl-Lys9 and acetylLys9 of Histone H3.

Immunogen

Peptide corresponding to amino acids surrounding Lys⁹ of human Histone H3, conjugated to KLH.

Application

Monoclonal Anti-unmodified-Histone H3 (Lys9) antibody produced in mouse has been used in:

• immunostaining
• immunoblotting
• immunofluorescence

Biochem/physiol Actions

Histones are subjected to extensive covalent modifications, including phosphorylation, methylation, acetylation and ubiquitination. These modifications are thought to play an important role in development and cancer. Histone H3 methylation at Lys9 is a modification intrinsically linked to epigenetic silencing and heterochromatin assembly. Histone methylation, as like acetylation, is a complex, dynamic process involving several processes, including transcriptional regulation, chromatin condensation, mitosis, and heterochromatin assembly. Moreover, lysine residues can be mono-, di-, and tri-methylated, adding further complexity to the regulation of chromatin structure.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage,freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable