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RAB0329

Sigma-Aldrich

Mouse SCF ELISA Kit

for serum, plasma and cell culture supernatant

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 2.74-2000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Kitl(17311)

General description

The Mouse SCF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse SCF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse SCF

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Stem cell Factor (SCF) or kit ligand or steel factor or mast cell growth factor is a growth factor essential to bring about various homeostatic events like maintenance of hematopoietic stem cells, mast cells and melanocytes. SCF is essential for maintaining mature melanocytes and to promote hair pigmentation. In ovary, granulosa cells produce kit ligand, which promotes activation and growth of primordial follicles. Kit ligand converts primordial follicles to primary follicles and leads to subsequent follicle growth. Kit ligand acts as an anti-apoptotic factor and causes the growth of primordial follicles in mouse fetal ovary. Kit signalling is crucial for growth of interstitial cells of Cajal (ICCs) and regulates gastrointestinal motility in fetal mice.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kit ligand decreases the incidence of apoptosis in cultured vitrified whole mouse ovaries.
Abdi S
Reproductive Biomedicine Online, 30(5), 493-503 (2015)
Stem cell factor/Kit signal insufficiency contributes to hypoxia-induced intestinal motility dysfunctions in neonatal mice
Ren H, et al.
Digestive Diseases and Sciences, 62(5), 1193-1203 (2017)
Charlotte B Westbury et al.
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, 111(2), 206-211 (2014-04-22)
Mast cells are involved in the pathogenesis of radiation fibrosis and may be a therapeutic target. The mechanism of increased mast cell number in relation to acute and late tissue responses in human skin was investigated. Punch biopsies of skin
Identification of hair shaft progenitors that create a niche for hair pigmentation
Liao CP, et al.
Genes & Development, 31, 1-13 (2017)
Armelle Calipel et al.
Clinical & experimental metastasis, 31(5), 553-564 (2014-03-22)
Imatinib mesylate is used in targeted therapy of cancer to inhibit type III tyrosine kinase receptors, such as KIT and platelet-derived growth factor receptors (PDGFRs). Expression of KIT in uveal melanoma (UM) suggests that this receptor may be the target
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