We do not have a pre-formulated protocol available at the moment. However, for reference, you may find the following publication informative. The publication, "Limited proteolysis of pyranose 2-oxidase results in a stable and active complex" in PeerJ Materials Science 2:e7 (https://doi.org/10.7717/peerj-matsci.7), served as the basis for this assay, utilizing the production of quinoneimine dye to directly reflect the turnover rate of pyranose oxidase.
P4234
Pyranose Oxidase from Coriolus sp.
recombinant, expressed in E. coli, ≥2.7 units/mg solid
Synonym(s):
Pyranose: Oxygen 2-Oxidoreductase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
powder
specific activity
≥2.7 units/mg solid
shipped in
wet ice
storage temp.
−20°C
General description
Pyranose oxidase (P2O), a homotetrameric protein consists of a covalently bound flavin adenine dinucleotide (FAD). It is seen mostly among wood-degrading basidiomycetes.[1]
Application
Biochem/physiol Actions
Pyranose oxidase (P2O) can be used in clinical chemistry to determine 1,5-anhydro-d-glucitol marker, used for glycemic control in diabetes patients.[1]
Pyranose oxidase (P2O) catalyzes the oxidation of aldopyranoses at position C-2 to yield the corresponding 2-ketoaldoses. The in vivo substrates of P2O are thought to be D-glucose, D-galactose, and D-xylose. They are oxidized to 2-keto-D-glucose (D-arabino-hexos-2-ulose, 2-dehydro-D-glucose), 2-keto-D-galactose (D-lyxo-hexos-2-ulose, 2-dehydro-D-galactose), and 2-keto-D-xylose (D-threopentos-2-ulose, 2-dehydro-D-xylose), respectively. Pyranose oxidase has significant activity with carbohydrates such as, L-sorbose, D-glucono-1,5-lactone, and D-allose. When pyranose oxidase catalyzes the oxidation of aldopyranoses, electrons are transferred to molecular oxygen which results in the formation of hydrogen peroxide[4].
Unit Definition
One unit produces 1.0 μmol of hydrogen peroxide per minute at 37 °C, pH 7.0.
Other Notes
Contains glutamate
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Tien-Chye Tan et al.
Journal of molecular biology, 409(4), 588-600 (2011-04-26)
Trametes multicolor pyranose 2-oxidase (P2O) is a flavoprotein oxidase that oxidizes d-glucose at C2 to 2-keto-d-glucose by a highly regioselective mechanism. In this work, fluorinated sugar substrates were used as mechanistic probes to investigate the basis of regioselectivity in P2O.
Purification and characterization of pyranose oxidase from the white rot fungus Trametes multicolor.
C Leitner et al.
Applied and environmental microbiology, 67(8), 3636-3644 (2001-07-27)
We purified an intracellular pyranose oxidase from mycelial extracts of the white rot fungus Trametes multicolor by using ammonium sulfate fractionation, hydrophobic interaction, ion-exchange chromatography, and gel filtration. The native enzyme has a molecular mass of 270 kDa as determined
Mor R Alkaslasi et al.
Nature communications, 12(1), 2471-2471 (2021-05-02)
In vertebrates, motor control relies on cholinergic neurons in the spinal cord that have been extensively studied over the past hundred years, yet the full heterogeneity of these neurons and their different functional roles in the adult remain to be
Wouter Van Hecke et al.
Bioresource technology, 100(23), 5566-5573 (2009-07-15)
The interactions between two oxidoreductases coupled by an artificial redox mediator have been described quantitatively to increase both stability and productivity. In this cascade oxidation, pyranose 2-oxidase oxidizes several aldoses at the C-2 position to 2-ketoaldoses. A redox mediator is
Oliver Spadiut et al.
The FEBS journal, 277(13), 2892-2909 (2010-06-10)
Pyranose 2-oxidase from Trametes multicolor is a 270 kDa homotetrameric enzyme that participates in lignocellulose degradation by wood-rotting fungi and oxidizes a variety of aldopyranoses present in lignocellulose to 2-ketoaldoses. The active site in pyranose 2-oxidase is gated by a
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Do you have a protocol for the activity assay used to qualify pyranose oxidase?
Do you have a protocol for the activity assay used to qualify pyranose oxidase?
1 answer-
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What is the solution stability for product P4234 after dilution?
1 answer-
Prepare stock solutions in 0.1 M Tris-HCl, pH 7.0. While no specific concentration was provided, calculations based on the assay procedure suggest that stock solutions should be in the range of 0.5 - 1.0 mg/ml protein. This aligns with the general recommendation for proteins of a minimum of 1 mg protein/ml. The stock solution can be stored as aliquots at -20°C and should be reasonably stable for several months.
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