The GALS kit, identified by product number F1797, is not tested or recommended for use with a mouse brain that has been fixed using a different fixative for 4 days or longer. The standard kit procedure fixes cells for only 10 minutes, whereas the fixation period used for the mouse brain is 396 times longer than what is recommended in the kit protocol. The kit was never tested for use on a mouse brain fixed using another fixative. Therefore, it is uncertain whether the fixation used for the mouse brain might produce acceptable results
GALS
β-Galactosidase Reporter Gene Staining Kit
Synonym(s):
β-galactosidase, LacZ
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.32
Pricing and availability is not currently available.
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usage
kit sufficient for 100 tests (using a 3.5 cm dish)
Quality Level
technique(s)
microbe id | staining: suitable
shipped in
dry ice
storage temp.
−20°C
General description
β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole.[1] The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide.[1] The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system.[2] The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.
Application
Other Notes
The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
Supplementary Hazards
Storage Class Code
3 - Flammable liquids
Flash Point(F)
132.8 °F
Flash Point(C)
56 °C
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Is the GALS kit compatible with fixed, frozen mouse brain that used a totally different fixative for 4 days or longer?
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