A3157

Alcian Blue 8GX

Dye content, ≥50%, certified by the Biological Stain Commission, powder

• Synonym(s): Alcian Blue, Ingrain Blue 1
• Empirical Formula (Hill Notation): C₅₆H₆₈Cl₄CuN₁₆S₄
• CAS Number: 33864-99-2
• Molecular Weight: 1298.86
• Colour Index Number: 74240
• EC Number: 251-705-7
• MDL number: MFCD00010720
• UNSPSC Code: 12171500
• PubChem Substance ID: 24890721
• NACRES: NA.47

Properties

• product name: Alcian Blue 8GX, certified by the Biological Stain Commission
• grade: certified by the Biological Stain Commission
• form: powder
• composition: Dye content, ≥50%
• color: dark blue
• solubility: water: 1 mg/mL, blue
• εmax: 195-400 at 617-630 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C
• InChI: 1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4
• InChI key: CKLBXIYTBHXJEH-UHFFFAOYSA-J

Description

Application

Alcian Blue 8GX has been used for the staining of sulfated proteoglycans in murine MPC (mesenchymal progenitor cell) nodule. It has been used for the staining of sulfated proteoglycans in bovine articular chondrocytes.

Biochem/physiol Actions

Alcian Blue 8GX is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby helping in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.

Suitability

Certified for use in staining in Mowry′s pH 2.5 method, in Scott′s pH 5.7 method, and in Kreyberg′s method for keratin and mucin.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves