11371
Avidin-Peroxidase Conjugate from hen egg white/horseradish
lyophilized, powder, almost white, avidin ~5 U/mg
Synonym(s):
Avidin-POD
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
form
powder
quality
lyophilized
avidin activity
~5 U/mg
impurities
≥80 U/mg peroxidase (further activity)
color
almost white
storage temp.
−20°C
Unit Definition
1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5 further activity: peroxidase ≥80 U/mg [1U corresponds 1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C]
Preparation Note
Conjugate Description: ~1.5 moles peroxidase/mol avidin; MBS-linkage (Cat. No.No. 63173)
Other Notes
Staining immunoblots with biotinylated primary antibodies
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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H.J. Willison et al.
IRCS Med. Sci., 14, 201-201 (1986)
E Lacey et al.
Analytical biochemistry, 163(1), 151-158 (1987-05-15)
A sensitive method for the nonisotopic in vitro labeling of proteins under physiological conditions using photobiotin, a compound originally developed for labeling nucleic acids (Forster et al. (1985) Nucleic Acids Res. 13, 745), has been developed. Using sheep brain tubulin
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