SCC190

J14 Human SLP-76-Deficient Jurkat Cell Line

• UNSPSC Code: 41106514
• eCl@ss: 32011203
• NACRES: NA.45

Properties

• description: Cell line type: Blood Cell
• technique(s): cell culture | mammalian: suitable

Description

General description

J14 cells are a unique Jurkat-derived human T cell line that lacks expression of SLP-76 (SH2 domain–containing leukocyte protein of 76 kilodaltons), an adaptor protein and PTK substrate expressed in hematopoietic cells (2). SLP-76 together with the scaffolding protein LAT act as a hub for various signaling effectors (1). Except SLP-76, the expression profile of J14 cells encompasses all necessary components of downstream pathways including those required for IL-2 cytokine induction (3), making the J14 cell line ideal for mutational analysis and introduction of reporter constructs.

Source:
J14 cells are a clonal derivative of Jurkat, a T cell line derived from bone marrow of a 14-year old male (4). Jurkat cells were transfected with an episomal vector and selected for hygromycin resistance. The transgene was subsequently lost via non-selective growth (2).

T-cell-receptor signaling has a critical role in adaptive immune responses and has been extensively studied as a general model for complex cellular responses (1). T-cell-receptor signaling to downstream pathways is mediated by non-receptor protein tyrosine kinases (PTKs), which interact with substrate proteins in a complex network.

References:
1. J Cell Sci. 2009; 122(Pt 9): 1269-1273.
2. J Biol Chem. 1995; 270(13): 7029-7032.
3. Science. 1998; 281(5375): 413-416.
4. Int J Cancer. 1977; 19(5): 621-626.

Application

This product is sold solely for research use per the terms of the “Restricted Use Agreement” which govern its use as detailed in the product Data Sheet or Certificate of Analysis. For information regarding any other use, please contact licensing@emdmillipore.com.

Quality

• Each vial contains >=1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): does not flash
• Flash Point(C): does not flash