MAB2137

Anti-Homing Receptor Antibody, clone A020

clone A020, Chemicon^®, from rat

• Synonym(s): CD44s, HCAM, Pgp-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: A020, monoclonal
• species reactivity: mouse, human, rat, pig, rabbit, canine, bovine
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2b
• NCBI accession no.: NM_000610.3; NM_001001389.1; NM_001001390.1; NM_001001391.1; NM_001001392.1
• UniProt accession no.: P16070
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... CD44(960)

Description

Specificity

Antibody reacts with the standard CD44 isoform (CD44s) that lacks exon sequences v1-v10. Also called human CD44 (85-95 kDa) gp90{Hermes} and mouse gp85 (Pgp-1).

Immunogen

Purified human blood lymphocyte CD44

Application

Anti-Homing Receptor Antibody, clone A020 is an antibody against Homing Receptor for use in ELISA, FC, IC, IH & WB.

ELISA 1:2000

Flow Cytometry :1:1000

Immunofluorescence 1:200

Immunohistostaining of paraffin embedded tissues. 1:250; antigen retrieval is usually not needed.

Immunoprecipitation 1:200

Western blotting: non-reduced conditions only; 90kDa band is detected; blocking PBS/5% milk protein, primary antibody dilution 1:1000 o/n incubation.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Target description

90 kDa

Physical form

Format: Purified

Liquid in 10 mM sodium phosphate, pH 7.5 with 150 mM NaCl and 0.01% sodium azide.

Protein A purified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Cells of epithelium and highly expressed by carcinomas

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable