AB3209
Anti-Oct-4 Antibody
Chemicon®, from rabbit
Synonym(s):
POU5F1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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![Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2, Alexa Fluor™ 488 conjugate clone 7F9.2, from mouse, ALEXA FLUOR™ 488](/deepweb/assets/sigmaaldrich/product/images/405/633/324511fc-ecc5-4c49-9d9d-8cf25ab28d40/640/324511fc-ecc5-4c49-9d9d-8cf25ab28d40.jpg)
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
input
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type: mouse embryonic stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... POU5F1(5460)
General description
Expression of the POU-domain transcription factor Octamer-4 (Oct-4) is widely regarded as a hallmark of pluripotent stem cells. The relationship of Oct-4 to pluripotent stem cells is indicated by its tightly restricted expression to undifferentiated pluripotent stem cells. Upon differentiation to somatic lineages, the expression of Oct-4 disappears rapidly. Unlike the majority of pluripotent stem cell markers, the biological role of Oct-4 has been well characterized. Studies performed in mice point to the critical role of Oct-4 in the establishment and/or maintenance of pluripotent stem cells in an uncommitted state.
Specificity
Recognizes Oct4 (POU5F1). The molecular weight of the protein is 38,601 Daltons.
Immunogen
Synthetic peptide, amino acids 282-297of Oct4.
Application
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
This Anti-Oct-4 Antibody is validated for use in IC, IH, WB for the detection of 41186.
Western blot: 1:200-1:1,000 using ECL. Oct-4 in cells typically shows ~40-44kDa band or doublet.
Immunohistochemistry: 1:200-1:1,000.
Immunocytochemistry: 1:200-1:1,000.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:200-1:1,000.
Immunocytochemistry: 1:200-1:1,000.
Optimal working dilutions must be determined by the end user.
Target description
44 kDa
Physical form
Affinity Purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
ImmunoAffinity Purified
Storage and Stability
Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.
For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
The rehydrated antibody solutions can be stored undiluted at 2–8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2–8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.
For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Analysis Note
Control
Pluripotent human embryonic stem (ES) cells
Pluripotent human embryonic stem (ES) cells
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Max Villa et al.
Biomedical microdevices, 12(2), 253-261 (2009-12-17)
We present optimal perfusion conditions for the growth of primary mouse embryonic fibroblasts (mEFs) and mouse embryonic stem cells (mESCs) using a microfluidic perfusion culture system. In an effort to balance nutrient renewal while ensuring the presence of cell secreted
Filipa Vlahova et al.
Scientific reports, 9(1), 8126-8126 (2019-06-01)
Expression of OCT4A is one of the hallmarks of pluripotency, defined as a stem cell's ability to differentiate into all the lineages of the three germ layers. Despite being defined as non-tumorigenic cells with high translational potential, human mid-trimester amniotic
Chawalit Siriboon et al.
PloS one, 10(2), e0118165-e0118165 (2015-02-14)
We attempted to isolate ES cell lines using inner cell masses from high-quality cloned porcine blastocysts. After being seeded onto feeders, embryos had better (P < 0.05) attachment, outgrowth formation and primary colonization in both 2× and 3× aggregated cloned
Oct4 mediates Müller glia reprogramming and cell cycle exit during retina regeneration in zebrafish.
Poonam Sharma et al.
Life science alliance, 2(5) (2019-10-09)
Octamer-binding transcription factor 4 (Oct4, also known as Pou5F3) is an essential pluripotency-inducing factor, governing a plethora of biological functions during cellular reprogramming. Retina regeneration in zebrafish involves reprogramming of Müller glia (MG) into a proliferating population of progenitors (MGPCs)
Nicole Renee Lee Sparks et al.
Stem cells (Dayton, Ohio), 36(3), 349-362 (2017-12-02)
Human pluripotent stem cell-derived osteoblasts possess great potential for use in bone disorder elucidation and repair; however, while the general ability of human pluripotent stem cells to differentiate into osteoblasts and lay down bone-specific matrix has been shown, previous studies
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