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Key Documents

375095

Millipore

Heparin, Sodium Salt, Porcine Intestinal Mucosa

Synonym(s):

Heparin, Sodium Salt, Porcine Intestinal Mucosa, HEP, Na

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About This Item

CAS Number:
9041-08-1
MDL number:
MFCD00081689
UNSPSC Code:
12352201
NACRES:
NA.79
Pricing and availability is not currently available.

description

Merck USA index - 14, 4653

Quality Level

100

form

lyophilized

specific activity

≥160 USP units/mg dry wt

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤0.5 EU/mg Endotoxin

solubility

water: 50 mg/mL

shipped in

ambient

storage temp.

15-25°C

SMILES string

[Na+].[S](=O)(=O)(NC1C(OC(C(C1O[S](=O)(=O)O)OC4OC(C(C(C4O[S](=O)(=O)O)O)O)C(=O)O)CO)OC2C(OC(C(C2O)O[S](=O)(=O)O)OC3C(OC(C(C3O)NC(=O)C)O)CO[S](=O)(=O)O)C(=O)O)O

InChI

1S/C26H42N2O37S5.Na/c1-4(30)27-7-9(31)13(6(56-23(7)39)3-55-67(43,44)45)58-26-19(65-70(52,53)54)12(34)16(20(62-26)22(37)38)60-24-8(28-66(40,41)42)15(63-68(46,47)48)14(5(2-29)57-24)59-25-18(64-69(49,50)51)11(33)10(32)17(61-25)21(35)36;/h5-20,23-26,28-29,31-

InChI key

JRTRSJGZMRQDHI-UHFFFAOYSA-N

General description

M.W. 13,500-15,000. Note: 1 KU = 1000 units.
MW: 15,000 ± 2,000
Preservative-free, non-sterile. Highly sulfated dextrorotatory mucopolysaccharide with specific anticoagulant properties. Inhibits IP3-activated Ca2+ channel release of the endoplasmic reticulum. Reported to activate the ryanodine receptor. Note: 1 KU = 1000 units. MW: 15,000 ± 2,000.
Preservative-free, non-sterile. Highly sulfated dextrorotatory mucopolysaccharide with specific anticoagulant properties. Inhibits the IP3-activated Ca2+ channel release of the endoplasmic reticulum. Reported to activate the ryanodine receptor. Note: 1 KU = 1000 units.

Warning

Toxicity: Harmful (C)

Other Notes

Seuwen, K., and Boddeke, H.G. 1995. Br. J. Pharmacol.114, 1613.
Ehrlich, B.E., et al. 1994. Trends Pharmacol. Sci.15,145.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Enrique Gómez et al.
Journal of proteome research, 19(3), 1169-1182 (2020-01-25)
Metabolomics may identify biomarkers in blood that differentiate pregnant from open embryo recipients. Fresh and vitrified/warmed, in vitro-produced embryos were transferred to Holstein recipients (discovery group). Recipient blood plasma collected on Day-0 (estrus) and Day-7 (before embryo transfer) were analyzed
Junxin Wang et al.
Bioconjugate chemistry, 29(11), 3768-3775 (2018-10-04)
We recently reported a real-time method to measure heparin in human whole blood based on the photoacoustic change of methylene blue (MB). Intriguingly, the MB behaved unlike other "turn on" photoacoustic probes-the absorbance decreased as the photoacoustic signal increased. The

Questions

1–2 of 2 Questions  

  1. I plan to use heparin in Neurobasal medium for culturing GBM cells. Literature suggests 2 µg/mL, but I need the units/mL for the final concentration. Could you provide guidance on the correct units/mL for heparin?

    1 answer

    1. Product 375095 is assayed for activity on a batch to batch basis with a specification for this product ≥160 USP units/mg dry weight. The reported activity value for this product will be found on the certificate of analysis for a given lot. It is unclear as to what activity corresponds to the provided concentration that was indicated and only the authors of the literature citation would know. Certain research articles do provide information on the activity of heparin to supplement neurobasal medium (NBM). Please see the article below which states NBM can be supplemented with 100 U/uL heparin. This decision will be at the discretion of the end user.

      https://www.sciencedirect.com/science/article/pii/S2666166723004884

      Helpful?

  2. What should be the storage temperature after 100 000 units of heparin salt is dissolved in 100 mL of PBS 1x

    1 answer

    1. Sterile filtered heparin solutions remain stable for up to 2 years when stored at 2-8 °C, provided th. Autoclaving and freezing heparin solutions are not recommended. Please see the link below to review additional information available in the datasheet for similar product H3393:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/112/608/h3393pis.pdf

      Helpful?

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