04-001
Anti-JAK2 Antibody, clone 8E10.2
clone 8E10.2, Upstate®, from mouse
Synonym(s):
Janus kinase 2, Tyrosine kinase
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43
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biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
8E10.2, monoclonal
species reactivity
mouse, human, rat
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... JAK2(3717)
mouse ... Jak2(16452)
rat ... Jak2(24514)
General description
JAK2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerizes and translocates to the nucleus and bind to CIS elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.
Specificity
This antibody recognizes Jak2.
Immunogen
KLH-conjugated linear peptide.
Application
Western Blot Analysis: 0.5-1 μg/mL of this lot was used to detect Jak2 in L6 cell lysates.
Immunohistochemistry(Paraffin):
A previous lot of this antibody was used in immunohistochemistry(paraffin) staining with eptiope retrieval: Human Cancer Array.
Immunohistochemistry(Paraffin):
A previous lot of this antibody was used in immunohistochemistry(paraffin) staining with eptiope retrieval: Human Cancer Array.
Anti-JAK2 Antibody, clone 8E10.2 detects level of JAK2 & has been published & validated for use in WB, IH(P).
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunological Signaling
Immunological Signaling
Quality
Routinely evaluated by western blot on L6 cell lysate.
Target description
130 kDa
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
L6 cell lysate
L6 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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