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HPLC Analysis of Nucleosides on Ascentis® Express OH5 (HILIC Mode)

HPLC Analysis of Nucleosides on Ascentis® Express OH5 (HILIC Mode) application for HPLC

Materiales

Analytical column

Referencia del producto
Descripción
Precios

Ascentis® Express OH5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Referencia del producto
Descripción
Precios

Nucleosides Test Mix

in 1% sodium formate (varied conc.), analytical standard

Adenosine

Pharmaceutical Secondary Standard; Certified Reference Material

Disodium Guanylate

Pharmaceutical Secondary Standard; Certified Reference Material

Uridine

Pharmaceutical Secondary Standard; Certified Reference Material

Guanosine

Pharmaceutical Secondary Standard; Certified Reference Material

5′-Adenylic Acid

Pharmaceutical Secondary Standard; Certified Reference Material

Timidina

pharmaceutical secondary standard

Cytidine

certified reference material, pharmaceutical secondary standard

CONDITIONS

column

Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U)

mobile phase

[A] 5 mM ammonium acetate, pH 5.0 with acetic acid in 95:5, acetonitrile:water; [B] 5 mM ammonium acetate, pH 5.0 with acetic acid in 80:20, acetonitrile:water

gradient

0% B held for 1 min; to 100% B in 10 min; held at 100% B for 1 min

flow rate

0.3 mL/min

column temp.

25 °C

detector

UV, 250 nm

injection

2 μL

sample

10 - 100 μg/mL in 95:5, acetonitrile:water (pH of buffer stock (in water) was adjusted before further dilution with water and/or acentonitrile)

Descripción

Analysis Note

Nucleosides are involved in many aspects of metabolism. Analogs of nucleosides have medical application in antivirals or anticancer treatments. This application demonstrates the utility of hydrophilic interaction chromatography (HILIC) for the retention and resolution of nucleosides and analogs thereof. Elution is not solely explained by logD but by other ionic and solvophobic effects.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany